...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Hydroxyapatite accelerates differentiation and suppresses growth of MC3T3-E1 osteoblasts
【24h】

Hydroxyapatite accelerates differentiation and suppresses growth of MC3T3-E1 osteoblasts

机译：羟基磷灰石加速分化并抑制MC3T3-E1成骨细胞的生长

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hydroxyapatite describes both the natural mineral phase of bone as well as the widely used calcium-phosphate implant substitute. Given that hydroxyapatite is a major component of the in vivo surface with which osteoblasts interact, it is surprising that most studies examining the regulation of osteoblast growth and differentiation utilize plastic surfaces. Here we demonstrate that the phenotype of mouse MC3T3-E1 osteoblasts is significantly altered on hydroxyapatite compared with plastic surfaces. Specifically, alkaline phosphatase activity and messenger RNA levels, markers of early stages of osteoblast differentiation, are increased in osteoblasts cultured on hydroxyapatite. The precocious appearance of alkaline phosphatase activity on the hydroxyapatite surface suggests that osteoblast differentiation is activated earlier compared with plastic surfaces. Osteocalcin expression, a marker of late-stage differentiation, is also increased on hydroxyapatite and further demonstrates enhanced differentiation. Cell counts indicate that fewer osteoblasts are present on hydroxyapatite versus plastic surfaces 24 h after plating. Measurement of osteoblast attachment, apoptosis, and necrosis indicated no differences between surfaces. In contrast, the number of bromode-oxyuridine-incorporating cells was significantly decreased on hydroxyapatite compared with plastic surfaces. Taken together, our findings indicate that hydroxyapatite enhances osteoblast differentiation while also suppressing growth.
机译：羟基磷灰石描述了骨骼的天然矿物质相以及广泛使用的磷酸钙植入物替代物。鉴于羟基磷灰石是成骨细胞与之相互作用的体内表面的主要成分，令人惊讶的是，大多数研究成骨细胞生长和分化调控的研究都利用了塑料表面。在这里，我们证明了与塑料表面相比，羟基磷灰石上小鼠MC3T3-E1成骨细胞的表型显着改变。具体而言，在羟磷灰石上培养的成骨细胞中，碱性磷酸酶活性和信使RNA水平（成骨细胞分化早期的标志物）增加。羟基磷灰石表面早熟的碱性磷酸酶活性表明，与塑料表面相比，成骨细胞分化被更早地激活。骨钙素表达是晚期分化的标志，在羟基磷灰石上也有所增加，并进一步证明了分化的增强。细胞计数表明，电镀后24小时，与塑料表面相比，羟基磷灰石上存在的成骨细胞更少。对成骨细胞附着，凋亡和坏死的测量表明表面之间没有差异。相比之下，与塑料表面相比，羟基磷灰石上掺入溴模式-氧尿苷的细胞数量明显减少。两者合计，我们的发现表明，羟基磷灰石增强成骨细胞分化，同时也抑制生长。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号