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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants
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Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants

机译：带有嵌套壳聚糖海绵的三维大孔磷酸钙生物陶瓷，用于承重骨植入物

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Three-dimensional macroporous calcium phosphate bioceramics embedded with porous chitosan sponges were synthesized to produce composite scaffolds with high mechanical strength and a large surface/volume ratio for load-bearing bone repairing and substitutes. The macro-porous calcium phosphate bioceramics with pore diameters of 300 mu m to 600 mu m were developed using a porogen burnout technique, and the chitosan sponges were formed inside the pores of the bioceramics by first introducing chiosan solution into the pores followed by a freeze-drying process. Our scanning electron microscopy results showed that the pore size of chitosan sponges formed inside the macro-porous structure of bioceramics was approximately 100 mu m, a structure favorable for bone tissue in-growth. The compressive modulus and yield stress of the composite scaffolds were both greatly improved in comparison with that of HA/beta-TCP scaffolds. The simulated body fluid (SBF) and cell culture experiments were conducted to assess the bioactivity and biocompatibility of the scaffolds. In the SBF tests, a layer of randomly oriented needle-like apatite crystals formed on the scaffold surface after sample immersion in SBF, which suggested that the composite material has good bioactivity. The cell culture experiments showed that MG63 osteoblast cells attached to the composite scaffolds, proliferated on the scaffold surface, and migrated onto the pore walls, indicating good cell biocompatibility of the scaffold. The cell differentiation on the composite scaffolds was evaluated by alkaline phosphatase (ALP) assay. Compared with the control in tissue culture dishes, the cells had almost the same ALP activity on the composite scaffolds during the first 11 days of culture.
机译：合成了嵌有多孔壳聚糖海绵的三维大孔磷酸钙生物陶瓷，以生产具有高机械强度和大表面积/体积比的复合支架，用于承重骨修复和替代物。利用致孔剂燃尽技术开发了孔径为300μm至600μm的大孔磷酸钙生物陶瓷，并通过先将壳聚糖溶液引入孔中然后冷冻，在生物陶瓷的孔内形成了脱乙酰壳多糖海绵。 -干燥过程。我们的扫描电子显微镜结果表明，在生物陶瓷大孔结构内部形成的壳聚糖海绵的孔径约为100微米，这种结构有利于骨组织的向内生长。与HA /β-TCP支架相比，复合支架的压缩模量和屈服应力均得到了极大的改善。进行了模拟体液（SBF）和细胞培养实验，以评估支架的生物活性和生物相容性。在SBF测试中，将样品浸入SBF后，在支架表面上形成了一层随机取向的针状磷灰石晶体，这表明该复合材料具有良好的生物活性。细胞培养实验表明，附着在复合支架上的MG63成骨细胞在支架表面上增殖并迁移到孔壁上，表明支架具有良好的细胞生物相容性。通过碱性磷酸酶（ALP）分析评估复合支架上的细胞分化。与组织培养皿中的对照相比，在培养的前11天中，这些细胞对复合支架的ALP活性几乎相同。

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