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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Engineering vascular networks in porous polymer matrices
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Engineering vascular networks in porous polymer matrices

机译：多孔聚合物基质中的工程血管网络

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Enhanced vascularization is critical to the treatment of ischemic tissues and the engineering of new tissues and organs. We have investigated whether sustained and localized delivery of vascular endothelial growth factor (VEGF) combined with transplantation of human microvascular endothelial cells (HMVECs) can be used to engineer new vascular networks. VEGF was incorporated and released in a sustained manner from porous poly (lactic-co-glycolic acid) (PLG) matrices to promote angiogenesis at the transplantation site. VEGF could be incorporated and released in a biologically active form from PLG matrices, with the majority of VEGF release (64 percent) occurring within 2 weeks. These matrices promoted a 260 percent increase in the density of host SCID mouse-derived capillaries invading the matrices after 7 days of implantation, confirming the activity of the released VEGF. HMVECs were transplanted into SCID mice on PLG matrices, and organized to form immature human-derived vessels within 3 days. Functional vessels were observed within 7 days. Importantly, when HMVECs were transplanted on VEGF-releasing matrices, a 160 percent increase in the density of human-derived blood vessels was observed after 14 days. These findings suggest that combining elements of vasculogenesis and angiogenesis provides a viable and novel approach to enhancing local vascularization.
机译：增强的血管形成对于缺血组织的治疗以及新组织和器官的工程设计至关重要。我们已经研究了是否可以持续和局部递送血管内皮生长因子（VEGF）并结合人微血管内皮细胞（HMVECs）的移植来设计新的血管网络。掺入VEGF并从多孔聚乳酸-乙醇酸共聚物（PLG）基质中持续释放，以促进移植部位的血管生成。可以掺入VEGF并以生物活性形式从PLG基质中释放，其中大部分VEGF释放（64％）发生在2周内。植入7天后，这些基质促进了宿主SCID小鼠衍生的毛细管侵袭基质的密度增加了260％，从而确认了释放的VEGF的活性。将HMVECs移植到PLG基质上的SCID小鼠中，并组织成在3天内形成未成熟的人源性血管。在7天内观察到功能性血管。重要的是，将HMVECs移植到可释放VEGF的基质上后，在14天后观察到人源血管密度增加了160％。这些发现表明，将血管生成和血管生成的要素相结合提供了一种增强局部血管形成的可行且新颖的方法。

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