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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Isolation of intact aortic valve scaffolds for heart-valve bioprostheses: Extracellular matrix structure, prevention from calcification, and cell repopulation features
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Isolation of intact aortic valve scaffolds for heart-valve bioprostheses: Extracellular matrix structure, prevention from calcification, and cell repopulation features

机译：用于心脏瓣膜生物假体的完整主动脉瓣支架的分离：细胞外基质结构，防止钙化和细胞重新聚集的功能

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Extracellular matrix (ECM) scaffolds isolated from valvulated conduits can be useful in developing durable bioprostheses by tissue engineering provided that anatomical shape, architecture, and mechanical properties are preserved. As evidenced by SEM, intact scaffolds were derived from porcine aortic valves by the combined use of Triton X-100 and cholate (TRI-COL) or N-cetylpyridinium (CPC) and subsequent nucleic acid removal by nuclease. Both treatments were effective in removing most cells and all the cytomembranes, with preservation of (1) endothelium basal membranes, (2) ECM texture, including the D-periodical interaction of small proteoglycans with normally D-banded collagen fibrils, and (3) mechanical properties of the treated valves. Ultrastructural features agreed with DNA, hexosamine, and uronic acid biochemical estimations. Calcification potential, assessed by a 6-week rat subdermal model, was significantly reduced by TRI-COLuclease treatment. This was not true for CPC only, despite better proteoglycan preservation, suggesting that nucleic acids also are involved in calcification onset. Human fibroblasts, used to repopulate TRI-COL samples, formed mono- or multilayers on surfaces, and groups of cells also were scattered within the valve leaflet framework. A biocompatible scaffolds of this kind holds promise for production of durable valve bioprostheses that will be able to undergo probable turnover and/or remodeling by repopulating recipient cells.
机译：从瓣膜导管中分离的细胞外基质（ECM）支架可用于通过组织工程开发耐用的生物假体，前提是保留解剖学形状，结构和机械特性。 SEM证明，完整的支架是通过结合使用Triton X-100和胆酸盐（TRI-COL）或N-鲸蜡基吡啶鎓（CPC）并随后通过核酸酶去除核酸而从猪主动脉瓣获得的。两种处理均能有效去除大多数细胞和所有细胞膜，同时保留（1）内皮基底膜，（2）ECM质地，包括小蛋白聚糖与正常D带胶原纤维的D周期相互作用，以及（3）处理过的阀门的机械性能。超微结构特征与DNA，己糖胺和糖醛酸生化估计一致。通过6周的大鼠皮下模型评估的钙化潜力通过TRI-COL /核酸酶处理显着降低。尽管蛋白聚糖保留得更好，但仅CPC并非如此，这表明核酸也参与钙化的开始。人成纤维细胞，用于重新填充TRI-COL样品，在表面形成单层或多层，细胞群也散布在瓣膜小叶框架内。这种生物相容性支架有望生产耐用的瓣膜生物假体，该假体将能够通过重新填充受体细胞而经历可能的周转和/或重塑。

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