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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Bone regeneration by grafting of an autogenous cultured bone/ceramic construct
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Bone regeneration by grafting of an autogenous cultured bone/ceramic construct

机译：通过移植自体培养的骨/陶瓷构建体进行骨再生

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摘要

The in vivo osteogenic potential of autogenous cultured bone/ceramic constructs in large animals or humans is unknown, and thus we performed a preliminary study of this issue prior to clinical application. All autogenous cultured-bone/ceramic constructs at 3 weeks after implantation in dogs showed obvious histological bone formation within the ceramic pores. In many pores, the HE staining of decalcified specimens revealed thick lamellar bone formation on the pore surface of ceramic. On the surface of bone tissue, numerous active cuboidal osteoblasts were evident. Biochemically, high alkaline phosphatase activity was detected in all dogs. Histological examination of the constructs at 8 weeks postimplantation showed lamellar bone formation with vascular system invasion into the pores, and regenerated hematopoietic bone marrow was often detected in association with the new bone in grafting of human cultured bone/ceramic constructs. Trilineage hematopoietic cells (i.e., granulocytic, erythroblastic, and megakaryocytic cells) were identified in the ceramic pores. Biochemically, high alkaline phosphatase activity and significant human osteocalcin content was detected in the constructs. Based on these findings, in the near future, this technique (grafting of patient-derived cultured bone/HA constructs) will be able to be applied to various bone reconstruction surgical treatments.
机译：在大型动物或人类中，自体培养的骨/陶瓷构建体的体内成骨潜力尚不清楚，因此，我们在临床应用之前对该问题进行了初步研究。犬植入后第3周，所有自体骨/陶瓷构建体在陶瓷孔内均显示出明显的组织学骨形成。在许多孔中，脱钙标本的HE染色显示陶瓷孔表面上有厚的层状骨形成。在骨组织表面，可以看到大量活跃的立方成骨细胞。生化方面，在所有狗中均检测到高碱性磷酸酶活性。植入后第8周对构建体进行组织学检查，发现层状骨形成，血管系统侵入毛孔，在人工培养的骨/陶瓷构建体移植中，经常会检测到再生造血骨髓和新骨。在陶瓷孔中鉴定出三谱系造血细胞（即粒细胞，红细胞和巨核细胞）。生化方面，在构建物中检测到高碱性磷酸酶活性和显着的人骨钙素含量。基于这些发现，在不久的将来，该技术（患者来源的培养的骨/ HA构建体的移植）将能够应用于各种骨重建手术治疗。

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