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首页> 外文期刊>Journal of Biomolecular Structure and Dynamics >Coexistence of G-quadruplex and duplex domains within the secondary structure of 31-mer DNA thrombin-binding aptamer
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Coexistence of G-quadruplex and duplex domains within the secondary structure of 31-mer DNA thrombin-binding aptamer

机译：31-mer DNA凝血酶结合适体二级结构中G-四链体和双链体结构域的共存

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摘要

A number of thrombin-binding DNA aptamers have been developed during recent years. So far the structure of just a single one, 15-mer thrombin-binding aptamer (15TBA), has been solved as G-quadruplex. Structures of others, showing variable anticoagulation activities, are still not known yet. In this paper, we applied the circular dichroism and UV spectroscopy to characterize the temperature unfolding and conformational features of 31-mer thrombin-binding aptamer (31TBA), whose sequence has a potential to form G-quadruplex and duplex domains. Both structural domains were monitored independently in 31TBA and in several control oligonucleotides unable to form either the duplex region or the G-quadruplex region. The major findings are as follows: (1) both duplex and G-quadruplex domains coexist in intramolecular structure of 31TBA, (2) the formation of duplex domain does not change the fold of G-quadruplex, which is very similar to that of 15TBA, and (3) the whole 31TBA structure disrupts if either of two domains is not formed: the absence of duplex structure in 31TBA abolishes G-quadruplex, and vice versa, the lack of G-quadruplex folding results in disallowing the duplex domain.
机译：近年来，已经开发了许多凝血酶结合DNA适体。到目前为止，只有一个15-mer凝血酶结合适体（15TBA）的结构已被解析为G-四链体。尚显示未知的其他抗凝血活性结构。在本文中，我们应用了圆二色性和紫外光谱来表征31-mer凝血酶结合适体（31TBA）的温度展开和构象特征，该序列具有形成G-四链体和双链体结构域的潜力。在31TBA和无法形成双链体区域或G-四链体区域的几个对照寡核苷酸中独立监测两个结构域。主要发现如下：（1）31TBA的分子内结构同时存在双链体和G-四链体域;（2）双链体域的形成不会改变G-四链体的折叠，这与15TBA的非常相似（3）如果未形成两个结构域中的任何一个，则整个31TBA结构将被破坏：31TBA中不存在双链体结构将消除G-quadruplex，反之亦然，缺少G-quadruplex折叠将导致不允许双链结构域。

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