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首页> 外文期刊>Journal of Biomolecular Structure and Dynamics >Long range molecular wire behaviour in a metal complex of DNA.
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Long range molecular wire behaviour in a metal complex of DNA.

机译：DNA金属络合物中的远距离分子线行为。

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摘要

M-DNA is a complex of metal ions such as Zn(2+) with duplex DNA. Previous results showed that the fluorescence of a donor fluorophore was quenched when an acceptor fluorophore was placed at the opposite end of a short M-DNA duplex. In order to investigate further the molecular wire behaviour of M-DNA, 30-mer duplexes were constructed with fluorescein as donor and rhodamine, pyrene and the cyanine dyes, Cy5 and Cy5.5 as acceptors. Good quenching was observed in all cases even though the efficiency of resonance energy transfer was calculated to be < 5%. The distance dependence of quenching was investigated by preparing doubly-labelled duplexes ranging in length from 20 to 1,000 base pairs. Upon formation of M-DNA significant quenching of the fluorescence of the donor fluorophore was observed in duplexes up to 500 base pairs in length. The amount of quenching decreased with increasing length of the duplexes with a shallow distance dependence. The results are consistent with an electron transfer mechanism in which the electron hops between metal centers. This process can occur efficiently over long distances.
机译：M-DNA是金属离子（例如Zn（2+））与双链DNA的复合物。先前的结果表明，将受体荧光团置于短M-DNA双链体的另一端时，供体荧光团的荧光被淬灭。为了进一步研究M-DNA的分子线行为，以荧光素为供体，若丹明，pyr和花青染料为Cy5和Cy5.5为受体，构建了30-mer双链体。即使计算得出的共振能量转移效率<5％，在所有情况下均观察到良好的淬灭。通过制备长度在20至1,000个碱基对之间的双标记双链体，研究了淬灭的距离依赖性。在形成M-DNA后，在长达500个碱基对的双链体中观察到供体荧光团的荧光的显着猝灭。淬灭的量随着双链体长度的增加而减小，并具有较浅的距离依赖性。结果与电子转移机制一致，电子在金属中心之间跳跃。此过程可以有效地进行很长的距离。

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