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首页> 外文期刊>Journal of Cancer Research and Clinical Oncology >Expression of costimulatory molecules, B7-1 and B7-2 on human gastric carcinoma.
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Expression of costimulatory molecules, B7-1 and B7-2 on human gastric carcinoma.

机译：共刺激分子B7-1和B7-2在人胃癌中的表达。

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摘要

Costimulation of T cells via B7-1 and B7-2 molecules on a tumor has been shown to be important for eliciting cell-mediated antitumor immunity. We studied the surface expression of B7-1 and B7-2 in 24 cases of gastric carcinoma from the primary locus, 20 cases of metastatic carcinoma from malignant ascites, 20 cases of benign gastric mucosa and 7 gastric carcinoma cell lines by two-color flow cytometry with mAb CD80 and CD86. The B7-1 and B7-2 molecules were expressed by 6 cell lines, and 1 cell line showed the predominant expression of B7-2 but not B7-1. Almost all patients with primary gastric carcinoma and benign gastric mucosa showed high levels of expression of the B7-1 and B7-2, revealing approximately 40%-60% positive cells. However, the percentage of B7-1-positive cells of poorly differentiated primary carcinomas was significantly lower than that of well-differentiated carcinoma and normal mucosa (P < 0.01). Furthermore, all of the metastatic carcinoma cells revealed consistently very low or undetectable levels of expression of the B7-1 molecule, only 8% (mean) of cells being positive, despite showing higher levels of B7-2 expression. Thus, it seems likely that decreased or deleted expression of B7-1 correlates with the grade of tumor differentiation, tumor progression and metastasis. These results suggest that the B7-1 molecule on the gastric carcinoma bearing CD80+CD86+ is abrogated during tumor invasion and/or metastasis, and the tumor finally acquires the CD80-CD86+ phenotype. Consequently, inadequate B7-1 costimulation may contribute to the escape of tumors from destruction by the host's immune system.
机译：T细胞通过肿瘤上的B7-1和B7-2分子共刺激已被证明对于引起细胞介导的抗肿瘤免疫力很重要。我们通过双色流研究了B7-1和B7-2在24例原发性胃癌，20例恶性腹水转移性癌，20例良性胃黏膜和7种胃癌细胞系中的表面表达。用mAb CD80和CD86进行细胞计数。 B7-1和B7-2分子由6个细胞系表达，其中1个细胞系主要表达B7-2，但不表达B7-1。几乎所有患有原发性胃癌和良性胃粘膜的患者均显示B7-1和B7-2的高水平表达，并显示出约40％-60％的阳性细胞。然而，低分化原发癌的B7-1阳性细胞百分比显着低于高分化癌和正常黏膜（P <0.01）。此外，所有转移性癌细胞始终显示出非常低或无法检测到的B7-1分子表达水平，尽管显示出更高水平的B7-2表达，但只有8％（平均值）的细胞为阳性。因此，B7-1表达的减少或缺失似乎与肿瘤分化程度，肿瘤进展和转移有关。这些结果表明，在肿瘤侵袭和/或转移过程中，带有CD80 + CD86 +的胃癌上的B7-1分子被消除，并且肿瘤最终获得了CD80-CD86 +表型。因此，不充分的B7-1共刺激可能导致肿瘤逃脱宿主免疫系统的破坏。

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