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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Biomonitoring of biosurfactant production by green fluorescent protein-marked Bacillus subtilis W1012
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Biomonitoring of biosurfactant production by green fluorescent protein-marked Bacillus subtilis W1012

机译：绿色荧光蛋白标记的枯草芽孢杆菌W1012对生物表面活性剂生产的生物监控

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BACKGROUND: Biosurfactant production was investigated using two strains of Bacillus subtilis, one being a reference strain (B. subtilis 1012) and the other a recombinant of this (B. subtilis W1012) made able to produce the green fluorescent protein (GFP). RESULTS: Batch cultivations carried out at different initial levels of glucose (G_o) in the presence of 10 g L~(-1) casein demonstrated that the reference strain was able to release higher levels of biosurfactants in the medium at 5.0 < Go < 10 g L~(-1) (B_(max)=104-110 mg L~(-1)). The recombinant strain exhibited slightly lower levels of biosurfactants (B_(max) = 90-104 mg L~(-1)) but only at higher glucose concentrations (G_020 g L~(-1)). Under these nutritional conditions, the fluorescence intensity linked to the production of GFP was shown to be associated with the cell concentration even after achievement of the stationary phase. CONCLUSION: The ability of the genetically-modified strain to simultaneously overproduce biosurfactant and GFP even at low biomass concentration makes it an interesting candidate for use as a biological indicator to monitor indirectly the biosurfactant production in bioremediation treatments.
机译：背景：使用两种枯草芽孢杆菌菌株研究了生物表面活性剂的生产，一种菌株是参考菌株（枯草芽孢杆菌1012），另一种是重组菌株（枯草芽孢杆菌W1012），能够生产绿色荧光蛋白（GFP）。结果：在10 g L〜（-1）酪蛋白存在下，以不同的初始葡萄糖水平（G_o）进行的分批培养表明，该参考菌株能够在5.0

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