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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Fermentation of cheese whey powder solution to ethanol in apacked-column bioreactor: effects of feed sugar concentration
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Fermentation of cheese whey powder solution to ethanol in apacked-column bioreactor: effects of feed sugar concentration

机译：填充柱生物反应器中奶酪乳清粉溶液向乙醇的发酵：进料糖浓度的影响

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BACKGROUND: Cheese whey powder (CWP) is a concentrated source of lactose and other essential nutrients for ethanol fermentation. CWP solution containing different concentrations of total sugar was fermented to ethanol in an up-flow packed-column bioreactor (PCBR) at a constant hydraulic residence time (HRT) of 50 h. Total sugar concentration in the feed was varied between 50 and 200 g L~(-1) and a pure culture of Kluyveromyces marxianus was used for ethanol fermentation of lactose. Variations of ethanol and sugar concentrations with the height of the column and with the feed sugar concentration were determined. RESULTS: Ethanol concentration increased and total sugar decreased with the column height for all feed sugar contents. The highest effluent ethanol concentration (22.5 g L~(-1)) and ethanol formation rate were obtained with feed sugar content of 100 g L~(-1). Percentage sugar utilization decreased with increasing feed sugar content above 100 g L~(-1) yielding lower ethanol contents in the effluent. The highest ethanol yield coefficient (0.52 gE g~(-1) S) was obtained with a feed sugar content of 50 g L~(-1). Biomass concentration also decreased with column height, yielding low ethanol formation in the upper section of the column. CONCLUSION: The packed column bioreactor was found to be effective for ethanol fermentation from CWP solution. The optimum feed sugar content maximizing the effluent ethanol and the specific rate of ethanol formation was found to be 100 g L~(-1). High sugar content above 100 g L~(-1) resulted in low ethanol productivities due to high maintenance requirements.
机译：背景：干酪乳清粉（CWP）是乳糖和乙醇发酵所必需的其他重要营养素的浓缩来源。将含有不同浓度的总糖的CWP溶液在上流式填充柱生物反应器（PCBR）中以50小时的恒定水力停留时间（HRT）发酵为乙醇。饲料中的总糖浓度在50至200 g L〜（-1）之间变化，使用纯克鲁维酵母培养物进行乳糖的乙醇发酵。确定了乙醇和糖浓度随柱高和进料糖浓度的变化。结果：随着进料糖含量的增加，乙醇浓度增加，总糖减少。进料糖含量为100 g L〜（-1）时，最高出水乙醇浓度（22.5 g L〜（-1））和乙醇生成速率。随着进料糖含量增加到100 g L〜（-1）以上，糖利用率降低，出水乙醇含量降低。进料糖含量为50 g L〜（-1）时，乙醇产率系数最高（0.52 gE g〜（-1）S）。生物质浓度也随塔高而降低，从而在塔上部形成低乙醇。结论：填充柱生物反应器可有效地从CWP溶液中发酵乙醇。发现使出水乙醇最大化和乙醇形成的特定速率的最佳进料糖含量为100 g L〜（-1）。糖含量超过100 g L〜（-1）时，由于维护要求高，导致乙醇生产率低。

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