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首页> 外文期刊>Journal of Cell Science >A novel linker histone-like protein is associated with cytoplasmic filaments in Caenorhabditis elegans.
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A novel linker histone-like protein is associated with cytoplasmic filaments in Caenorhabditis elegans.

机译：一种新型接头组蛋白样蛋白与秀丽隐杆线虫的胞质细丝相关。

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摘要

The histone H1 complement of Caenorhabditis elegans contains a single unusual protein, H1.X. Although H1.X possesses the globular domain and the canonical three-domain structure of linker histones, the amino acid composition of H1.X is distinctly different from conventional linker histones in both terminal domains. We have characterized H1.X in C. elegans by antibody labeling, green fluorescent protein fusion protein expression and RNA interference. Unlike normal linker histones, H1.X is a cytoplasmic as well as a nuclear protein and is not associated with chromosomes. H1.X is most prominently expressed in the marginal cells of the pharynx and is associated with a peculiar cytoplasmic cytoskeletal structure therein, the tonofilaments. Additionally H1.X::GFP is expressed in the cytoplasm of body and vulva muscle cells, neurons, excretory cells and in the nucleoli of embryonic blastomeres and adult gut cells. RNA interference with H1.X results in uncoordinated and egg laying defective animals, as well asin a longitudinally enlarged pharynx. These phenotypes indicate a cytoplasmic role of H1.X in muscle growth and muscle function.
机译：秀丽隐杆线虫的组蛋白H1补体包含单个异常蛋白H1.X。尽管H1.X具有接头组蛋白的球形结构域和规范的三结构域结构，但H1.X的氨基酸组成在两个末端结构域中均与常规接头组蛋白明显不同。我们已经通过抗体标记，绿色荧光蛋白融合蛋白表达和RNA干扰表征了秀丽隐杆线虫中的H1.X。与正常接头组蛋白不同，H1.X是细胞质以及核蛋白，与染色体无关。 H1.X最明显地表达在咽的边缘细胞中，并与其中的特殊胞质细胞骨架结构，即扁桃丝相关。另外，H1.X :: GFP在人体和外阴肌肉细胞，神经元，排泄细胞的细胞质中以及在胚胎卵裂球和成年肠道细胞的核仁中表达。 RNA对H1.X的干扰会导致不协调和产卵的缺陷动物，以及纵向扩大的咽部。这些表型表明H1.X在肌肉生长和肌肉功能中的细胞质作用。

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