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首页> 外文期刊>Journal of Chromatography, Biomedical Applications >Simple high-performance liquid chromatography method for the simultaneous determination of serum caffeine and paraxanthine following rapid sample preparation
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Simple high-performance liquid chromatography method for the simultaneous determination of serum caffeine and paraxanthine following rapid sample preparation

机译：快速样品制备后同时测定血清咖啡因和对黄嘌呤的简单高效液相色谱方法

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摘要

A simple reversed-phase high-performance liquid chromatography (HPLC) method for the simultaneous determination of caffeine and paraxanthine in human serum is described. Serum proteins are precipitated with perchloric acid and the resulting supernatant neutralized for direct injection onto an HPLC column. The method uses a phosphate-methanol mobile phase (85:15, v/v) at pH 4.9 with a flow-rate of 1.75 ml/min and quantitation is by UV absorbance at 274 nm. Elution times are approximately 18 min for caffeine and 8 min for paraxanthine. Theobromine and theophylline have elution times of 5.4 and 9.4 min and do not interfere in the assay. The intra-assay and between-assay means for precision and accuracy for both drugs are: 4.5% C.V. and 3.3% deviation. The sensitivity of the method is 50 ng/ml for each drug. (C) 1998 Elsevier Science B.V. [References: 16]
机译：描述了一种同时测定人血清中咖啡因和对黄嘌呤的简单反相高效液相色谱（HPLC）方法。血清蛋白用高氯酸沉淀，所得的上清液被中和后直接注射到HPLC柱上。该方法使用pH值为4.9的磷酸盐-甲醇流动相（85:15，v / v），流速为1.75 ml / min，通过274 nm处的UV吸收进行定量。咖啡因的洗脱时间约为18分钟，对黄嘌呤的洗脱时间约为8分钟。可可碱和茶碱的洗脱时间分别为5.4和9.4分钟，并且不会干扰测定。两种药物的精密度和准确度的测定内和测定间手段是：4.5％C.V.和3.3％的偏差。该方法的灵敏度为每种药物50 ng / ml。 （C）1998 Elsevier Science B.V. [参考：16]

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