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首页> 外文期刊>Journal of Chromatography, Biomedical Applications >Reversed-phase high-performance liquid chromatographic assay method for quantitating 6-mercaptopurine and its methylated and non-methylated metabolites in a single sample
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Reversed-phase high-performance liquid chromatographic assay method for quantitating 6-mercaptopurine and its methylated and non-methylated metabolites in a single sample

机译：反相高效液相色谱法测定单个样品中的6-巯基嘌呤及其甲基化和非甲基化代谢物

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摘要

Methods of assaying 6-mercaptopurine (6MP) and its methylated and non-methylated metabolites are essential for the therapeutic dose in treating patients with acute lymphoblastic leukemia. However, previous methods are technically complicated and unsuitable for clinical use. Thus, we have now developed a method utilizing reversed-phase highperformance liquid chromatography (HPLC) in order to quantify these compounds in human red blood cells (RBCs) in a single sample to serve as an index of cytotoxic activity. The agents 6MP 6-thioguanine (6TG) and 6-methylmercaptopurine (6MMP) were well separated by this assay. Linear relationships were observed between the peak areas and the RBC concentrations of 6MP, 6TG and 6MMP over the range of 20-2000, 18-1800 and 18-1800 pmol per 25 mg hemoglobin (Hb), respectively. The limit of quantitation of the assay is 20, 18 and 18 pmol per 25 mg Hb, respectively. This assay system is suitable for routine clinical use. (C) 1998 Elsevier Science B.V. All rights reserved. [References: 15]
机译：测定6-巯基嘌呤（6MP）及其甲基化和非甲基化代谢物的方法对于治疗急性淋巴细胞白血病患者的治疗剂量至关重要。但是，先前的方法在技术上很复杂并且不适用于临床。因此，我们现在已经开发出一种利用反相高效液相色谱（HPLC）的方法，以便在单个样品中定量人类红细胞（RBC）中的这些化合物，以作为细胞毒性活性的指标。通过该测定法，试剂6MP 6-硫鸟嘌呤（6TG）和6-甲基巯基嘌呤（6MMP）可以很好地分离。在每25 mg血红蛋白（Hb）的20-2000、18-1800和18-1800 pmol范围内，分别观察到6MP，6TG和6MMP的峰面积与RBC浓度之间存在线性关系。该测定的定量极限分别为每25​​ mg Hb 20 pmol，18 pmol和18 pmol。该测定系统适合常规临床使用。 （C）1998 Elsevier Science B.V.保留所有权利。 [参考：15]

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