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首页> 外文期刊>Journal of Comparative Physiology, B. Biochemical, Systemic, and Environmental Physiology >Functional characterisation of UCP1 in the common carp: uncoupling activity in liver mitochondria and cold-induced expression in the brain
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Functional characterisation of UCP1 in the common carp: uncoupling activity in liver mitochondria and cold-induced expression in the brain

机译：UCP1在鲤鱼中的功能表征：肝线粒体中的解偶联活性和脑中冷诱导的表达

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摘要

Mammalian uncoupling protein 1 (UCP1) mediates nonshivering thermogenesis in brown adipose tissue. We previously reported on the presence of a UCP1 orthologue in ectothermic fish and observed downregulation of UCP1 gene expression in the liver of the common carp. Neither the function of UCP1, nor the mode of UCP1 activation is known in carp liver mitochondria. Here, we compared the proton conductance at 25 degrees C of liver mitochondria isolated from carp either maintained at 20 degrees C (warm-acclimated, WA) or exposed to 8 degrees C (cold-acclimated, CA) water temperature for 7-10 days. Liver mitochondria from WA carp had higher state four rates of oxygen consumption and greater proton conductance at high membrane potential. Liver mitochondria from WA, but not from CA, carp showed a strong increase in proton conductance when palmitate (or 4-hydroxy-trans-2-nonenal, HNE) was added, and this inducible proton conductance was prevented by addition of GDP. This fatty acid sensitive proton leak is likely due to the expression of UCP1 in the liver of WA carp. The observed biochemical properties of proton leak strongly suggest that carp UCP1 is a functional uncoupling protein with broadly the same activatory and inhibitory characteristics as mammalian UCP1. Significant UCP1 expression was also detected in our previous study in whole brain of the carp. We here observed a twofold increase of UCP1 mRNA in carp brain following cold exposure, suggesting a role of UCP1 in the thermal adaptation of brain metabolism. In situ hybridization located the UCP1 gene expression to the optic tectum responsible for visual system control, the descending trigeminal tract and the solitary tract. Taken together, this study characterises uncoupling protein activity in an ectotherm for the first time.
机译：哺乳动物解偶联蛋白1（UCP1）介导棕色脂肪组织中不发抖的生热作用。我们以前曾报道过在外热鱼类中存在UCP1直向同源物，并观察到鲤鱼肝脏中UCP1基因表达的下调。在鲤鱼肝线粒体中，UCP1的功能和UCP1激活的方式均未知。在这里，我们比较了分离自鲤鱼的肝线粒体在25摄氏度下的质子电导，该鲤鱼保持在20摄氏度（温适应，WA）或暴露于8摄氏度（冷适应，CA）的水温下7-10天。 WA鲤的肝线粒体在高膜电位下具有较高的状态四耗氧率和较高的质子传导率。来自华盛顿州而不是来自加利福尼亚州的肝线粒体，添加棕榈酸酯（或4-羟基-反式-2-壬烯醛，HNE）时，鲤鱼的质子电导显着增加，并且这种可诱导的质子电导被GDP所阻止。脂肪酸敏感的质子泄漏可能是由于WA鲤鱼肝脏中UCP1的表达。观察到的质子泄漏的生化特性强烈表明，鲤鱼UCP1是一种功能性解偶联蛋白，具有与哺乳动物UCP1大致相同的激活和抑制特性。在我们先前的研究中，鲤鱼全脑中也检测到了显着的UCP1表达。我们在这里观察到冷暴露后鲤鱼脑中UCP1 mRNA的两倍增加，表明UCP1在脑代谢的热适应中发挥了作用。原位杂交将UCP1基因表达定位于负责视觉系统控制，三叉神经下降道和孤立道的视神经皮层。两者合计，这项研究首次表征了在等温线中解偶联蛋白的活性。

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