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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Fluorometric determination of ammonium ion by ion chromatography using postcolumn derivatization with o-phthaldialdehyde
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Fluorometric determination of ammonium ion by ion chromatography using postcolumn derivatization with o-phthaldialdehyde

机译：邻苯二甲醛柱后衍生离子色谱荧光法测定铵离子

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摘要

A postcolumn fluorometric derivatization method for the determination of trace amounts of ammonium ion (mu g/L level) under matrices with high concentrations of sodium and amino acids has been developed. In this method, ammonium ion was determined by ion chromatography combined with fluorometric detection (IC-FL) in less than 16 min. IC was performed in a high-capacity cation-exchange Dionex IonPac CS 16 analytical column (250 rum x 5 mm) under isocratic conditions with 30 mM methanesulfonic acid (MSA) as mobile phase at flow-rate 1.0 mL/min. To remove amino acid interference, the postcolumn derivatization based on the reaction of ammonia with o-phthaldialdehyde (OPA) and sulfite was applied. The excitation and emission wavelengths were 364 and 425 nm, respectively. The effects of pH, reaction temperature and time, OPA-reagent composition and concentration, and sample matrix were studied. The linear range and detection limit of this method were similar to the standard method. The IC-FL method with a postcolumn fluorometric derivatization allows the routine determination of ammonium ion in extreme matrices where the ratios of sodium and amino acids to ammonium are up to 2800000:1 and 28000: 1, respectively. (c) 2005 Elsevier B.V. All rights reserved.
机译：已开发出一种柱后荧光衍生化方法，用于测定钠和氨基酸浓度较高的基质下的痕量铵离子（μg / L）。在此方法中，通过离子色谱结合荧光检测（IC-FL）在不到16分钟的时间内即可确定铵离子。在等容量条件下，以高容量阳离子交换Dionex IonPac CS 16分析柱（250 rum x 5 mm），以30 mM甲磺酸（MSA）作为流动相，以1.0 mL / min的流速进行IC分析。为了消除氨基酸干扰，应用了基于氨与邻苯二甲醛（OPA）和亚硫酸盐反应的柱后衍生。激发和发射波长分别为364和425 nm。研究了pH，反应温度和时间，OPA试剂组成和浓度以及样品基质的影响。该方法的线性范围和检出限与标准方法相似。柱后荧光衍生化的IC-FL方法允许常规测定极端基质中的铵离子，其中钠和氨基酸与铵的比率分别高达2800000：1和28000：1。 （c）2005 Elsevier B.V.保留所有权利。

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