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首页> 外文期刊>Journal of Controlled Release: Official Journal of the Controlled Release Society >Development of a gene-activated scaffold platformfor tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds
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Development of a gene-activated scaffold platformfor tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

机译：使用基于胶原的支架上的壳聚糖-DNA纳米粒子开发用于组织工程应用的基因激活支架平台

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Biomaterial scaffolds that support cell infiltration and tissue formation can also function as platforms for the delivery of therapeutics such as drugs, proteins, and genes. As burst release of supraphysiological quantities of recombinant proteins can result in adverse side effects, the objective of this study was to explore the potential of a series of collagen-based scaffolds, developed in our laboratory, as gene-activated scaffold platforms with potential in a range of tissue engineering applications. The potential of chitosan, a biocompatible material derived from the shells of crustaceans, as a gene delivery vector was assessed using mesenchymal stem cells (MSCs). A transfection efficiency of >45% is reported which is similar to what is achieved with polyethyleneimine (PEI), a non-viral gold standard vector, without causing cytotoxic side effects. When the optimised chitosan nanoparticles were incorporated into a series of collagen-based scaffolds, sustained transgene expression from MSCs seeded on the scaffolds was maintained for up to 28 days and interestingly the composition of the scaffold had an effect on transfection efficiency. These results demonstrate that by simply varying the scaffold composition and the gene (or combinations thereof) chosen; the systemhas potential for amyriad of therapeutic applications. (C) 2015 Elsevier B.V. All rights reserved.
机译：支持细胞浸润和组织形成的生物材料支架也可以用作递送药物，蛋白质和基因等治疗剂的平台。由于超生理量的重组蛋白的突然释放可能导致不良副作用，因此本研究的目的是探索在我们实验室中开发的一系列基于胶原的支架的潜力，将其作为基因激活的支架平台，并将其潜力组织工程应用范围。使用间充质干细胞（MSC）评估了壳聚糖（一种来自甲壳动物壳的生物相容性材料）作为基因传递载体的潜力。据报道转染效率> 45％，与使用非病毒金标准载体聚乙烯亚胺（PEI）所达到的转染效率相似，且不会引起细胞毒性副作用。当将优化的壳聚糖纳米颗粒掺入一系列基于胶原的支架中时，来自接种在支架上的MSC的持续转基因表达可以维持长达28天，有趣的是，支架的组成对转染效率有影响。这些结果表明，通过简单地改变支架组成和选择的基因（或其组合）;该系统具有无数治疗应用的潜力。 （C）2015 Elsevier B.V.保留所有权利。

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