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首页> 外文期刊>Journal of dermatological science >Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application.
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Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application.

机译：重复2,4,6-三硝基-1-氯苯（TNCB）引起的皮炎的小鼠模型分析。

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BACKGROUND: The model of chronic dermatitis caused by repeated application of hapten is frequently used as a tool for assessment of the efficacy of a compound or the elucidation of chronic dermatitis. OBJECTIVE: The purpose of this study is to provide more detailed analysis of the model of chronic dermatitis caused by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB). METHODS: BALB/c mice were sensitized with TNCB on day -7 to the ear, and then TNCB was repeatedly applied to the same ear three times per week, through days 0-21. RESULTS: The repeated application of TNCB induced an increase of ear thickness, and a relatively steep increment of ear thickness from days 7 to 9 was observed. This increment reached almost a plateau at day 9. The peaks of ear swelling on days 7, 14 and 21 were approximately two times higher than that on day 0. Regarding cytokines in the ear, the highest production of IL-1 beta, 4, 6 and 18 were observed on day 7. The peak production of IL-1 beta, 4 and 6 on day 7 was found within 24 h after the challenge, while that of IL-18 was found at 0 h. The inflammatory cell infiltration into epidermis and dermis was observed and increased in day 7, then reached almost a plateau on day 9. CONCLUSION: The indices such as thickness, swelling and inflammatory cell infiltration in the lesional skin was increased and maintained by repeated application, however, the protein levels of some cytokines were not always consistent with the reactions.
机译：背景：反复使用半抗原引起的慢性皮炎模型经常用作评估化合物功效或阐明慢性皮炎的工具。目的：本研究的目的是对因反复使用2,4,6-三硝基-1-氯苯（TNCB）引起的慢性皮炎模型进行更详细的分析。方法：在第-7天用TNCB对BALB / c小鼠的耳朵进行敏化，然后每周0至21天，每周3次将TNCB重复应用于同一只耳朵。结果：TNCB的重复使用引起了耳厚的增加，并且从第7天到第9天观察到耳厚相对较陡的增加。这种增加在第9天几乎达到平稳。在第7、14和21天，耳朵肿胀的峰值大约是在第0天的两倍。关于耳朵中的细胞因子，IL-1β的最高产量为4在第7天观察到了6和18。在攻击后24小时内发现了IL-1 beta，4和6的峰值产生，而在0小时时发现了IL-18的峰值产生。在第7天观察到炎症细胞向表皮和真皮的浸润并增加，然后在第9天几乎达到平稳状态。结论：通过反复使用，可以增加并保持病变皮肤中的厚度，肿胀和炎症细胞浸润等指标，然而，某些细胞因子的蛋白质水平并不总是与反应一致。

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