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首页> 外文期刊>Journal of dentistry >The efficacy of techniques for the disinfection of artificial sub-surface dentinal caries lesions and their effect on demineralization and remineralization in vitro.
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The efficacy of techniques for the disinfection of artificial sub-surface dentinal caries lesions and their effect on demineralization and remineralization in vitro.

机译：人造水下表面龋齿损伤消毒技术的功效及其对体外脱矿质和再矿化的影响。

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OBJECTIVES: The efficacy of three techniques for the disinfection of artificial sub-surface root caries lesions and their response to subsequent episodes of de- and remineralization was investigated quantitatively in vitro. METHODS: Sub-surface dentinal lesions (n=20), cut into four experimental blocks and deliberately contaminated with Streptococcus mutans, were subject to either steam autoclaving (121 degrees C, 5min), gamma irradiation (4100Gy), immersion in 0.1% (w/v) thymol-distilled water solution (24h) or reserved as a control. Next, the lesions were incubated aerobically in sterile nutrient broth for 24h at 37 degrees C and resultant cultures plated onto blood agar and neutralisation agar. Ten blocks from each experimental group were then immersed in an acidic buffer solution or exposed to artificial saliva for 5 days. Baseline changes in the mineral content and distribution of the lesions were assessed by transverse microradiography (TMR). RESULTS: Micro-organisms were recovered from each control block and one block treated by gamma irradiation. Steam autoclaving and immersion in a thymol solution significantly decreased (p<0.05) the amount of mineral lost from the body of lesions subject to a further acid challenge. Mineral ion uptake by lesions exposed to artificial saliva was significantly increased (p<0.05) through disinfection by steam autoclaving. CONCLUSIONS: Gamma irradiation proved the most acceptable method for the disinfection of sub-surface root dentine lesions having the least adverse effect on demineralization and remineralization.
机译：目的：在体外定量研究了三种技术对人造表面下根龋病变的消毒效果及其对随后脱矿质和再矿化的反应。方法：将表面亚表层牙本质病变（n = 20）切成四个实验块并故意受到变形链球菌污染，对其进行蒸汽高压灭菌（121℃，5min），γ射线照射（4100Gy），浸入0.1％（ w / v）百里香酚蒸馏水溶液（24h）或保留作为对照。接下来，将病灶在无菌营养肉汤中在37摄氏度有氧条件下培养24小时，并将所得培养物铺在血琼脂和中和琼脂上。然后将每个实验组的十个试块浸入酸性缓冲溶液中或暴露于人工唾液中5天。矿物质含量和病变分布的基线变化通过横向显微放射照相术（TMR）进行评估。结果：从每个对照块和一个经γ射线处理的块中回收了微生物。蒸汽高压灭菌和浸入百里香酚溶液中可显着降低（p <0.05）遭受酸攻击的病灶体内矿物质流失的数量。通过蒸汽高压灭菌消毒，暴露于人工唾液的病灶对矿物质离子的摄取显着增加（p <0.05）。结论：伽马射线辐射被证明是最可接受的表面亚表面牙本质牙本质消毒方法，对脱矿质和再矿化的不利影响最小。

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