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首页> 外文期刊>Journal of dermatological science >Analysis of T cell receptor (TCR) BV-gene clonotypes in NC/Nga mice developing dermatitis resembling human atopic dermatitis.
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Analysis of T cell receptor (TCR) BV-gene clonotypes in NC/Nga mice developing dermatitis resembling human atopic dermatitis.

机译：分析发展为类似于人异位性皮炎的皮炎的NC / Nga小鼠中T细胞受体（TCR）BV基因的克隆型。

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BACKGROUND: Our previous study showed that T cells in skin lesions of human atopic dermatitis (AD) had oligoclonal accumulation, indicating the involvement of antigen-specific immune reactions at those sites. Recently, NC/Nga mice, which develop skin lesions similar to AD, have been proposed as a model for that disease. OBJECTIVE: To clarify whether NC/Nga mice are suitable as a model for human AD from the viewpoint of their antigen-specific immune responses. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) and single strand conformation polymorphism (SSCP) analyses were conducted to detect TCR BV genes of clonally expanded T cells derived from NC/Nga mice at an early phase of the AD-like dermatitis, at a late phase of the dermatitis, and with no AD-like dermatitis. RESULTS: (1) T cells with TCR BV 7, 10 and 17 reside in the skin of NC/Nga mice without the AD-like dermatitis. (2) T cells with these BV genes contain oligoclonal accumulations, however, expanded T cell clonotypes are alsodetected in the spleen and exist constantly during the course of the AD-like dermatitis. (3) Development of the AD-like dermatitis is associated with additional oligoclonal expansion/accumulation of T cells with TCR BV 2, 4 and 6 genes. (4) Progression of the AD-like dermatitis is associated with further oligoclonal expansion/accumulation of T cells with the TCR BV 14 gene. (5) Some of the expanded TCR clonotypes are common between the individual mice and between early and late phases. CONCLUSIONS: Taking these data together with the previous human AD studies, NC/Nga mice seem to be an appropriate model for human AD.
机译：背景：我们先前的研究表明，人类特应性皮炎（AD）皮肤病变中的T细胞具有寡克隆积累，表明抗原特异性免疫反应参与了这些部位。近来，已经提出了发展类似于AD的皮肤损伤的NC / Nga小鼠作为该疾病的模型。目的：从抗原特异性免疫应答的角度，阐明NC / Nga小鼠是否适合作为人AD的模型。方法：进行逆转录聚合酶链反应（RT-PCR）和单链构象多态性（SSCP）分析，以检测AD样皮炎早期NC / Nga小鼠克隆扩增T细胞的TCR BV基因。 ，是在皮炎的晚期，没有AD样皮炎。结果：（1）具有TCR BV 7、10和17的T细胞存在于NC / Nga小鼠的皮肤中，而没有AD样皮炎。 （2）具有这些BV基因的T细胞含有寡克隆积累，但是，在脾脏中也检测到扩增的T细胞克隆型，并且在AD样皮炎的过程中不断存在。 （3）AD样皮炎的发展与具有TCR BV 2、4和6基因的T细胞的额外寡克隆扩增/积累有关。 （4）AD样皮炎的进展与TCR BV 14基因使T细胞进一步寡克隆扩增/积累有关。 （5）一些扩展的TCR克隆型在个体小鼠之间以及早期和晚期之间是常见的。结论：将这些数据与以前的人类AD研究一起考虑，NC / Nga小鼠似乎是人类AD的合适模型。

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