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首页> 外文期刊>Journal of dermatological science. Supplement >Cytokine profiles of skin lesions in murine lupus models
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Cytokine profiles of skin lesions in murine lupus models

机译：小鼠狼疮模型中皮肤病变的细胞因子谱

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Skin lesions of cutaneous lupus erythematosus (LE) have a number of infiltrating T cells, and these T cells and their related cytokines are involved in the development of skin lesions.In order to verify the roles of T cells and their related cytokines, we compared the cytokine profiles of LE-like skin lesions in experimentally-induced knock out (KO) mice and autoimmune-prone mice.Lesional skin samples were obtained from CD4 KO mice and TCRα KO mice of the B6 background treated with ultraviolet B light (UVBL) and fluorouracil (FU), and MRL/lpr mice. The levels of mRNAs for TNF-α, IFN-γ, IL-2, IL-4, IL-10 and IL-12 were determined by RT-PCR in these skin samples.Combination treatment with UVBL and FU to TCRα KO mice and CD4 KO mice revealed the importance of CD4+ CD8? γδT cells in the induction of drug induced cutaneous LE. Moreover, IL-12, IFN-γ and TNF-α were upregulated in TCRα KO mice treated with UVBL and FU. In contrast, IFN-γ was not elevated in the spontaneous LE-like skin lesions of MRL/lpr mice.Cytokine profile pattern varies from strain to strain, and that murine cutaneous LE models, like human LE patients, manifest a wide range of immunological abnormalities, including cytokines.
机译：皮肤红斑狼疮（LE）的皮肤病变有许多浸润性T细胞，这些T细胞及其相关的细胞因子参与了皮肤病变的发展。为了验证T细胞及其相关的细胞因子的作用，我们进行了比较实验性基因敲除（KO）小鼠和自身免疫易感小鼠中LE样皮肤病变的细胞因子谱。从皮肤B4背景的CD4 KO小鼠和TCRαKO小鼠获得紫外线B光（UVBL）处理后的皮肤病变样本和氟尿嘧啶（FU）和MRL / lpr小鼠。通过RT-PCR测定这些皮肤样本中TNF-α，IFN-γ，IL-2，IL-4，IL-10和IL-12的mRNA水平。 CD4 KO小鼠揭示了CD4 + CD8的重要性？ γδT细胞在药物诱导的皮肤LE中的诱导作用。此外，用UVBL和FU处理的TCRαKO小鼠中IL-12，IFN-γ和TNF-α上调。相比之下，MRL / lpr小鼠自发性LE样皮肤病变中IFN-γ并未升高，细胞因子的分布模式因品系而异，鼠类皮肤LE模型（如人类LE患者）表现出广泛的免疫学异常，包括细胞因子。

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