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首页> 外文期刊>Journal of experimental & clinical cancer research : >Preferential transcription of a gene for alpha amylase in the carcinoid tumor of African rodent Mastomys natalensis.
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Preferential transcription of a gene for alpha amylase in the carcinoid tumor of African rodent Mastomys natalensis.

机译：非洲啮齿类动物马斯特菌的类癌中α淀粉酶基因的优先转录。

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摘要

The carcinoid tumor in Mastomys natalensis provides a useful animal model of tumorigenesis. We investigated preferentially transcribed genes in this carcinoid tumor by differential hybridization. Fourteen clones corresponding to high levels of transcription were isolated from a cDNA library. Sequencing analysis and a homology search revealed that the clones corresponded to genes for chromogranin and alpha-amylase. High-level transcription of a gene for alpha-amylase gene in Mastomys carcinoid tumor was confirmed by Northern blotting analysis. Furthermore, Western blotting analysis confirmed the expression of alpha-amylase in tumors at the protein level. Immunohistochemical staining revealed alpha-amylase in the cytoplasm of Mastomys carcinoid tumors. Our results demonstrated that an exocrine enzyme 'amylase' could be produced ectopically by a neuroen docrine tumor.
机译：natalasts natalensis中的类癌肿瘤提供了有用的肿瘤发生动物模型。我们通过差异杂交研究了该类癌中优先转录的基因。从cDNA文库中分离出对应于高水平转录的十四个克隆。测序分析和同源性搜索表明，这些克隆对应于嗜铬粒蛋白和α-淀粉酶的基因。通过Northern印迹分析证实了Mastomys类癌肿瘤中α-淀粉酶基因的基因的高水平转录。此外，蛋白质印迹分析证实了蛋白质水平上肿瘤中α-淀粉酶的表达。免疫组织化学染色显示，Mastomys类癌肿瘤细胞质中存在α-淀粉酶。我们的结果表明，外分泌酶“淀粉酶”可以由神经元内分泌肿瘤异位产生。

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