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首页> 外文期刊>Journal of Experimental Botany >Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.
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Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.

机译：拟南芥韧皮部分泌物中脂质和脂质结合蛋白的鉴定。

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The phloem plays a crucial role in assimilate and nutrient transport, pathogen response, and plant growth and development. Yet, few species have yielded pure phloem exudate and, if proteins need to be analysed, those species may not have sequenced genomes, making identification difficult. The enrichment of Arabidopsis thaliana phloem exudate in amounts large enough to allow for metabolite and protein analysis is described. Using this method, it was possible to identify 65 proteins present in the Arabidopsis phloem exudate. The majority of these proteins could be grouped by response to pathogens, stress, or hormones, carbon metabolism, protein interaction, modification, and turnover, and transcription factors. It was also possible to detect 11 proteins that play a role in lipid/fatty acid metabolism (aspartic protease, putative 3-beta-hydroxysteroid dehydrogenase, UDP-sulphoquinovose synthase/SQD1, lipase, PIG-P-like protein: phosphatidylinositol-N-acetylglucosaminyltransferase), storage (glycine-rich protein), binding (annexin, lipid-associated family protein, GRP17/oleosin), and/or signalling (annexin, putative lipase, PIG-P-like protein). Along with putative lipid-binding proteins, several lipids and fatty acids could be identified. Only a few examples exist of lipids (jasmonic acid, oxylipins) or lipid-binding proteins (DIR1, acyl-CoA-binding protein) in the phloem. Finding hydrophobic compounds in an aqueous environment is not without precedence in biological systems: human blood contains a variety of lipids, many of which play a significant role in human health. In blood, lipids are transported while bound to proteins. The present findings of lipids and lipid-binding proteins in phloem exudates suggest that a similar long-distance lipid signalling exists in plants and may play an important role in plant growth and development.
机译：韧皮部在吸收和吸收养分，病原体反应以及植物生长发育中起着至关重要的作用。但是，只有极少数物种产生了韧皮部分泌液，如果需要分析蛋白质，这些物种可能没有基因组测序，因此很难鉴定。描述了足够大的数量足以进行代谢物和蛋白质分析的拟南芥韧皮部分泌液的富集。使用这种方法，有可能鉴定出拟南芥韧皮部分泌物中存在的65种蛋白质。这些蛋白质中的大多数可以按对病原体，应激或激素，碳代谢，蛋白质相互作用，修饰和更新以及转录因子的反应进行分组。还可以检测出11种在脂质/脂肪酸代谢中起作用的蛋白质（天冬氨酸蛋白酶，推定的3-β-羟基甾类脱氢酶，UDP-磺基奎诺糖合酶/ SQD1，脂肪酶，PIG-P-样蛋白：磷脂酰肌醇-N乙酰葡萄糖胺基转移酶），存储（富含甘氨酸的蛋白），结合（annexin，脂质相关的家族蛋白，GRP17 /油质蛋白）和/或信号传导（annexin，推定的脂肪酶，PIG-P样蛋白）。连同推定的脂质结合蛋白，可以鉴定出几种脂质和脂肪酸。韧皮部中仅存在脂类（茉莉酸，脂蛋白）或脂类结合蛋白（DIR1，酰基辅酶A结合蛋白）的几个例子。在水性环境中发现疏水性化合物并非没有生物系统的先例：人体血液中含有多种脂质，其中许多脂质对人体健康具有重要作用。在血液中，脂质在与蛋白质结合时被转运。韧皮部分泌物中脂质和脂质结合蛋白的当前发现表明植物中存在相似的长距离脂质信号传导，并且可能在植物生长和发育中起重要作用。

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