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首页> 外文期刊>Journal of Experimental Botany >Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei
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Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei

机译：RNA聚合酶II在拟南芥中间相核中的丰度和分布

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摘要

RNA polymerase II (RNAPII) is responsible for the transcription of most eukaryotic protein-coding genes. Analysing the topological distribution and quantification of RNAPII can contribute to understanding its function in interphase nuclei. Previously it was shown that RNAPII molecules in plant nuclei form reticulate structures within euchromatin of differentiated Arabidopsis thaliana nuclei rather than being organized in distinct 'transcription factories' as observed in mammalian nuclei. Immunosignal intensity measurements based on specific antibody labelling in maximum intensity projections of image stacks acquired by structured illumination microscopy (SIM) suggested a relative proportional increase of RNAPII in endopolyploid plant nuclei. Here, photoactivated localization microscopy (PALM) was applied to determine the absolute number and distribution of active and inactive RNAPII molecules in differentiated A. thaliana nuclei. The proportional increase of RNAPII during endopolyploidization is confirmed, but it is also shown that PALM measurements are more reliable than those based on SIM in terms of quantification. The single molecule localization results show that, although RNAPII molecules are globally dispersed within plant euchromatin, they also aggregate within smaller distances as described for mammalian transcription factories.
机译：RNA聚合酶II（RNAPII）负责大多数真核蛋白质编码基因的转录。分析RNAPII的拓扑分布和定量分析有助于理解其在相间核中的功能。以前已经证明，植物核中的RNAPII分子在分化的拟南芥核的常染色质中形成网状结构，而不是像哺乳动物核中那样在不同的“转录工厂”中组织。通过结构化照明显微镜（SIM）获得的图像堆栈最大强度投影中基于特异性抗体标记的免疫信号强度测量结果表明，内倍体植物细胞核中RNAPII相对比例增加。在这里，光活化的定位显微镜（PALM）用于确定分化的拟南芥核中活性和非活性RNAPII分子的绝对数量和分布。证实了内聚倍体化过程中RNAPII的比例增加，但也显示PALM测量在定量方面比基于SIM的测量更可靠。单分子定位结果表明，尽管RNAPII分子总体上分散在植物常染色质中，但它们也以较小的距离聚集，如哺乳动物转录工厂所述。

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