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首页> 外文期刊>Journal of Equine Veterinary Science >Acute altitude exposure (3800 meters) and metabolic capacity in the middle gluteal muscle of equids.
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Acute altitude exposure (3800 meters) and metabolic capacity in the middle gluteal muscle of equids.

机译：马的中间臀肌的急性高原暴露（3800米）和代谢能力。

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摘要

This study examined changes in citrate synthase (CS), beta-hydroxyacyl-CoA-dehydrogenase (HOAD), lactate dehydrogenase (LDH) and total protein (TP) activity of skeletal muscle in equids after acute high altitude exposure (3800 m). The middle glutealmuscles of trained equids (one Quarter Horse, one Shetland pony, and 4 Arabians) were sampled before altitude exposure (225 m) and after 9 days of sub-maximal exercise at altitude (3800 m). Muscle biopsies were take from a location 10 cm dorsocaudal tothe tuber coxae at an angle of 45o. The insertion site was standardized to a depth of 8 cm. Tissue homogenates were assayed for TP and maximal activity of CS, HOAD and LDH. All samples were run in duplicates and comparisons performed using a paired Student's t-test (significance set at P<0.05). Altitude did not change CS, HOAD or TP. CS for pre-altitude and altitude averaged 31.2±2.9 and 32.6±4.4μmolthing-1thinmin-1, respectively (P=0.51). HOAD values averaged 17.9±2.1 and 18.2±2.9μmolthing-1thinmin-1, respectively (P=0.85). Altitude acclimatization decreased LDH activity. Pre-altitude and altitude LDH averaged 725.4±43.4 and 672.7±51.5μmolthing-1thinmin-1, respectively (P=0.04). The decrease in LDH is consistent with decreases in skeletal muscle observed in other mammals at high altitude and suggests that muscles do not become more glycolytic in altitude hypoxia.
机译：这项研究检查了急性高海拔暴露（3800 m）后马匹中骨骼肌中柠檬酸合酶（CS），β-羟酰基辅酶A脱氢酶（HOAD），乳酸脱氢酶（LDH）和总蛋白（TP）活性的变化。在海拔高度之前（225 m）和海拔高度（3800 m）进行次最大运动9天后，对训练有素的马匹的中臀肌（四分之一马，一只设得兰群岛小马和四只阿拉伯人）进行采样。肌肉活检取自后尾10 cm处到块茎coxae处，成45度角。将插入部位标准化为8 cm的深度。测定组织匀浆的TP和CS，HOAD和LDH的最大活性。所有样品均一式两份运行，并使用配对的学生t检验进行比较（显着性设定为P <0.05）。海拔高度未更改CS，HOAD或TP。前高度和高度的CS平均分别为31.2±2.9和32.6±4.4μmolthing-1thinmin-1（P = 0.51）。 HOAD平均值分别为17.9±2.1和18.2±2.9μmolthing-1thinmin-1（P = 0.85）。海拔高度适应降低了LDH活性。海拔高度前的LDH平均值分别为725.4±43.4和672.7±51.5μmolthing-1thinmin-1（P = 0.04）。 LDH的减少与在高海拔地区其他哺乳动物中观察到的骨骼肌减少相一致，这表明在低氧状态下，肌肉的糖酵解作用不会增强。

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