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首页> 外文期刊>Journal of chemotherapy >Rapid susceptibility test for Mycobacterium tuberculosis to isoniazid and rifampin with resazurin method in screw-cap tubes.
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Rapid susceptibility test for Mycobacterium tuberculosis to isoniazid and rifampin with resazurin method in screw-cap tubes.

机译：用刃天青法在螺帽管中快速检测结核分枝杆菌对异烟肼和利福平的敏感性。

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摘要

Multi-drug resistant (MDR) Mycobacterium tuberculosis is still a serious public health problem all over the world. MDR tuberculosis (MDR-TB) caused by these strains has emerged within the last decade and rapid detection is critical for the effective treatment of patients. Recently, a resazurin microtiter assay plate for detecting MDR strains was developed. In this study, it was adapted to screw-cap tubes and the activity of isoniazid (INH) and rifampin (RIF) to 50 M. tuberculosis clinical isolates was tested by this method for the first time. Results were compared with the radiometric reference method for the susceptibility testing of M. tuberculosis complex. The results of both methods were in 100% and 96% agreement for RIF and INH, respectively. Specificity, sensitivity, positive predictive value and negative predictive value were 91.7%, 100%, 92.8% and 100% for INH, respectively. All of these values were 100% for RIF. Susceptibility testing results were obtained on the 8th day of incubation for 42 isolates and on the 9th day for the other eight strains. Our results indicate that this method is suitable for the early determination of INH and RIF resistance in developing countries because it is inexpensive, rapid and easy to perform.
机译：耐多药（MDR）结核分枝杆菌仍然是世界范围内的严重公共卫生问题。由这些菌株引起的耐多药结核病（MDR-TB）在过去十年中已经出现，快速检测对于有效治疗患者至关重要。近来，开发了用于检测MDR菌株的刃天青微量滴定测定板。在这项研究中，它适用于螺旋盖管，并首次通过这种方法测试了异烟肼（INH）和利福平（RIF）对50 M结核病临床分​​离株的活性。将结果与放射线参考方法进行结核分枝杆菌复合物药敏试验进行比较。两种方法的结果分别为RIF和INH的100％和96％一致。 INH的特异性，敏感性，阳性预测值和阴性预测值分别为91.7％，100％，92.8％和100％。对于RIF，所有这些值均为100％。在孵育的第8天获得了42个分离株的敏感性测试结果，在其他8个菌株的第9天获得了敏感性测试结果。我们的结果表明，该方法便宜，快速且易于执行，因此适合于发展中国家中INH和RIF耐药的早期测定。

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