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首页> 外文期刊>Journal of genetics >Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci
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Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci

机译：Glu-A1和Glu-B1位点含无效等位基因的合成六倍体小麦的品质

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摘要

Triticum turgidum ssp. dicoccon PI94668 and PI349045 were identified as containing null alleles at Glu-A1 and Glu-B1 loci in previous investigation. Sequencing of the respective HMW-GS genes Ax, Bx, Ay and By in both accessions indicated equal DNA lengths with gene silencing caused by 1 to 4 in-frame stop codon(s) in the open reading frames. Six synthetic hexaploid wheat lines were produced by crossing PI94668 or PI349045 with six Aegilops tauschii by spontaneous chromosome doubling of unreduced gametes. As expected, these amphiploids had three different HMW-GS: Dx 3.1~t + Dy11*~t, Dx2.1~t + 10~t and Dx2~t + Dy12~t in Glu-D1 but double nulls in Glu-A1 and Glu-B1. Quality tests showed that most quality parameters in two T. turgidum ssp. dicoccon parents were very low due to the lack of HMW-GSs. However, incorporation of HMW-GS from Ae. tauschii in six synthetic hexaploid wheat lines significantly increased most quality related parameters. The potential values of these wheat lines in improving the quality of wheat are discussed.
机译：小麦白粉菌在先前的研究中，dicoccon PI94668和PI349045被鉴定为在Glu-A1和Glu-B1基因座上包含无效等位基因。在两个登录号中，各自的HMW-GS基因Ax，Bx，Ay和By的测序均表明相等的DNA长度，且由开放阅读框中的1至4个框内终止密码子引起的基因沉默。通过使未减配配子的自发染色体倍增，使PI94668或PI349045与六只节节紫锥菊杂交，产生了六种合成六倍体小麦品系。正如预期的那样，这些二倍体在Glu-D1中具有三种不同的HMW-GS：Dx 3.1〜t + Dy11 *〜t，Dx2.1〜t + 10〜t和Dx2〜t + Dy12〜t，但在Glu-A1中为双无效和Glu-B1。质量测试表明，大多数质量参数位于两个T. turgidum ssp。由于缺乏HMW-GS，双三叶草的父母非常低。但是，并入了Ae的HMW-GS。六个合成六倍体小麦品系中的tauschii显着提高了大多数与品质相关的参数。讨论了这些小麦品系在改善小麦品质方面的潜在价值。

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