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首页> 外文期刊>Journal of genetics >Genomewide identification and expression analysis of the ARF gene family in apple
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Genomewide identification and expression analysis of the ARF gene family in apple

机译:苹果ARF基因家族的全基因组鉴定和表达分析

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摘要

Auxin response factors (ARF) are transcription factors that regulate auxin responses in plants. Although the genomewide analysis of this family has been performed in some species, little is known regarding ARF genes in apple (Malus domestica). In this study, 31 putative apple ARF genes have been identified and located within the apple genome. The phylogenetic analysis revealed that MdARFs could be divided into three subfamilies (groups I, II and III). The predicted MdARFs were distributed across 15 of 17 chromosomes with different densities. In addition, the analysis of exon-intron junctions and of the intron phase inside the predicted coding region of each candidate gene has revealed high levels of conservation within and between phylogenetic groups. Expression profile analyses of MdARF genes were performed in different tissues (root, stem, leaf, flower and fruit), and all the selected genes were expressed in at least one of the tissues that were tested, which indicated that MdARFs are involved in various aspects of physiological and developmental processes of apple. To our knowledge, this report is the first to provide a genomewide analysis of the apple ARF gene family. This study provides valuable information for understanding the classification and putative functions of the ARF signal in apple.
机译:生长素应答因子(ARF)是调节植物中生长素应答的转录因子。尽管已经在某些物种中对该家族进行了全基因组分析,但对于苹果(Malus domestica)中的ARF基因知之甚少。在这项研究中,已经鉴定出31个假定的苹果ARF基因并将其定位在苹果基因组内。系统发育分析表明,MdARFs可以分为三个亚家族(I,II和III组)。预测的MdARFs分布在17个具有不同密度的染色体中的15个中。另外,对每个候选基因的预测编码区域内的外显子-内含子连接和内含子相的分析表明,在系统发生进化组内和之间的保守程度很高。在不同的组织(根,茎,叶,花和果实)中进行了MdARF基因的表达谱分析,所有选择的基因均在至少一种被测试的组织中表达,这表明MdARFs涉及各个方面。的生理和发育过程据我们所知,该报告是第一个提供苹果ARF基因家族全基因组分析的报告。这项研究为理解苹果中ARF信号的分类和假定功能提供了有价值的信息。

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