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首页> 外文期刊>Journal of genetics >Mutant allele of rna14 in fission yeast affects pre-mRNA splicing
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Mutant allele of rna14 in fission yeast affects pre-mRNA splicing

机译：裂变酵母中rna14的突变等位基因影响前mRNA剪接

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摘要

Spliceosome and 3-end processing complexes are necessary for the precursor mRNA (pre-mRNA) maturation. Spliceosome complex removes noncoding introns, while 3-end processing involves in cleavage and addition of poly(A) tails to the nascent transcript. Rna14 protein in budding yeast has been implicated in cleavage and polyadenylation of mRNA in the nucleus but their role in the pre-mRNA splicing has not been studied. Here, we report the isolation of a mutant allele of rna14 in fission yeast, Schizosaccharomyces pombe that exhibits reduction in protein level of Chk1 at the nonpermissive temperature, primarily due to the defects in posttranscriptional processing. Reverse transcriptase-polymerase chain reaction analysis reveals defective splicing of the chk1 (+) transcript at the nonpermissive temperature. Apart from chk1 (+), the splicing of some other genes were also found to be defective at the nonpermissive temperature suggesting that Rna14 might be involved in pre-mRNA splicing. Subsequently, genetic interaction of Rna14 with prp1 and physical interactions with Prp28 suggest that the Rna14 might be part of a larger protein complex responsible for the pre-mRNA maturation.
机译：剪接体和3末端加工复合物是前体mRNA（pre-mRNA）成熟所必需的。剪接体复合物可去除非编码内含子，而3末端加工涉及对新生转录物的切割和添加poly（A）尾巴。 Rna14蛋白在发芽酵母中已经参与了细胞核中mRNA的切割和聚腺苷酸化，但尚未研究其在mRNA剪接前的作用。在这里，我们报道了裂变酵母裂殖酵母中rna14突变等位基因的分离，该裂殖酵母在非许可温度下表现出Chk1蛋白水平降低，这主要是由于转录后加工中的缺陷所致。逆转录酶-聚合酶链反应分析显示在不允许的温度下，chk1（+）转录物的剪接有缺陷。除了chk1（+），在非许可温度下还发现其他一些基因的剪接有缺陷，这提示Rna14可能参与了mRNA的剪接。随后，Rna14与prp1的遗传相互作用以及与Prp28的物理相互作用表明，Rna14可能是负责mRNA预成熟的较大蛋白复合物的一部分。

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