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首页> 外文期刊>Journal of genetics >Biallelic expression of Tssc4, Nap1l4, Phlda2 and Osbpl5 in adult cattle
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Biallelic expression of Tssc4, Nap1l4, Phlda2 and Osbpl5 in adult cattle

机译：Tssc4，Nap1l4，Phlda2和Osbpl5在成年牛中的双等位基因表达

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摘要

Genomic imprinting of the Cdkn1c/Kcnq1ot1 region shows lack of conservation between human and mouse. This region has been reported to be associated with Beckwith-Wiedemann syndrome (BWS) and cancer. To increase our understanding of imprinted genes in bovine Cdkn1c/Kcnq1ot1 imprinting cluster, we assessed the imprinting status of four cattle genes (Tssc4, Nap1l4, Phlda2 and Osbpl5) in seven types of tissues: heart, liver, spleen, lung, kidney, skeletal muscle and subcutaneous fat using polymorphism-based sequencing approach. It was found that all the four genes showed biallelic expression in tissues in which transcripts were detected. Nap1l4 and Tssc4 were detected in all examined tissues, while the expression of Phlda2 and Osbpl5 was tissue-specific. Phlda2 was not detected in heart and subcutaneous fat, and Osbpl5 was not detected in spleen and skeletal muscle. In addition, identification of species-specific imprinted genes is necessary to understand the evolution of genomic imprinting and to elucidate mechanisms leading to allele-specific expression.
机译：Cdkn1c / Kcnq1ot1区的基因组印记显示人与小鼠之间缺乏保守性。据报道该区域与贝克威斯-韦德曼综合症（BWS）和癌症有关。为了增加我们对牛Cdkn1c / Kcnq1ot1印迹簇中印迹基因的理解，我们评估了四种牛基因（Tssc4，Nap1l4，Phlda2和Osbpl5）在七种类型的组织（心脏，肝脏，脾脏，肺，肾，骨骼）中的印迹状态肌肉和皮下脂肪使用基于多态性的测序方法。发现所有四个基因在检测到转录本的组织中均显示双等位基因表达。在所有检查的组织中均检测到Nap1l4和Tssc4，而Phlda2和Osbpl5的表达是组织特异性的。在心脏和皮下脂肪中未检测到Phlda2，在脾脏和骨骼肌中未检测到Osbpl5。另外，鉴定物种特异性的印迹基因对于理解基因组印迹的演变以及阐明导致等位基因特异性表达的机制是必要的。

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