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首页> 外文期刊>Journal of genetics >Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid
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Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid

机译：rDNA基因的染色体定位和5S rDNA在莫桑比克罗非鱼，乌拉尔角horn及其杂交种中的基因组结构

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摘要

In this study, classical and molecular cytogenetic analyses were performed in tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O. urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing O. mossambicus female × O. u. hornorum male. An identical karyotype ((2n = 44, NF (total number of chromosomal arms) = 50) was obtained from three examined tilapia samples. Genomic organization analysis of 5S rDNA revealed two different types of 5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals on six chromosomes of all the analysed metaphases from the three tilapia samples. Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence signals were observed on three chromosome pairs in all analysed metaphases of the three tilapia samples. The correlation between 45 rDNA localization and nucleolar organizer regions (NORs) was confirmed by silver nitrate staining in tilapia fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were verified by two different colour FISH probes. Briefly, the current data provide an insights for hybridization projects and breeding improvement of tilapias.
机译：本研究通过罗非鱼（O.chromos mossambicus）（XX / XY性别确定系统），O.urolepis hornorum（WZ / ZZ性别确定系统）及其杂交，对罗非鱼（O.urolepis hornorum）及其杂种进行了经典和分子细胞遗传学分析。你角质男。从三个罗非鱼样品中获得了相同的核型（（2n = 44，NF（染色体臂总数）= 50）。5S rDNA的基因组组织分析显示了两种不同类型的5S rDNA序列，即5S I型和5S II型。此外，用5S rDNA探针进行的荧光原位杂交（FISH）在来自三个罗非鱼样品的所有被分析中期的六个染色体上均显示了六个阳性荧光信号，随后还制备了45S rDNA探针，并在六个罗非鱼样品上观察到六个阳性荧光信号。在三个罗非鱼样品的所有已分析中期中均存在三个染色体对，通过硝酸银染色在罗非鱼中证实了45 rDNA定位与核仁组织区的相关性，并通过5S rDNA和45S rDNA的染色体定位进行了验证。两种不同的彩色FISH探针。简而言之，当前数据为罗非鱼的杂交项目和育种改良提供了见识。

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