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首页> 外文期刊>Journal of Food Lipids >Lipase-catalyzed acidolysis of algal oils with capric acid: Optimization of reaction conditions using response surface methodolgy
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Lipase-catalyzed acidolysis of algal oils with capric acid: Optimization of reaction conditions using response surface methodolgy

机译：用癸酸进行的脂肪酶对藻油的酸解：使用响应面法优化反应条件

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摘要

Lipase-assisted acidolysis of algal oils, arachidoinc acid single cell oil (ARASCO), docosahexaenoic acid single cell oil (DHASCO) and a single cell oil rich in both docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA, n-6) known as OMEGA-GOLD, with a medium-chain fatty acid (capric acid) was studied. Response surface methodology was used to obtain a maximum incorporation of CA into algal oils. The process variables studied were the amount of enzyme (2-6 %), reaction temperature (35-55C) and incubation time (12-36h). The amount of water added and mole ratio of substrate (algal oil to CA) were kept at 2 % and 1:3, respectively. All experiments were conducted according to a face-centered cube design. Under optimum conditions (12.3% of enzyme; 45C; 29.4 h), the incorporation of CA was 20.0% into ARASCO, 22.6% into DHASCO (4.2% enzyme; 43.3C 27.1 h) and 20.7% into the OMEGA-GOLD oil (2.5% enzyme, 46.6C 25.2 h).
机译：脂肪酶对藻油，花生四烯酸单细胞油（ARASCO），二十二碳六烯酸单细胞油（DHASCO）和富含二十二碳六烯酸（DHA）和二十二碳五烯酸（DPA，n-6）的单细胞油的酸解研究了具有中链脂肪酸（癸酸）的OMEGA-GOLD。响应面方法用于最大程度地将CA掺入藻油中。研究的过程变量是酶的量（2-6％），反应温度（35-55C）和孵育时间（12-36h）。加水量和底物的摩尔比（藻油与CA）分别保持在2％和1：3。所有实验均根据面心立方体设计进行。在最佳条件下（12.3％的酶; 45°C; 29.4小时），CA掺入ARASCO的比例为20.0％，DHASCO的掺入率为22.6％（4.2％的酶; 43.3C的比例为27.1 h），OMEGA-GOLD油的掺入率为20.7％（2.5 ％酶，46.6℃25.2小时）。

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