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首页> 外文期刊>Biochimica et biophysica acta. Molecular basis of disease: BBA >Increased aquaporin 1 and 5 membrane expression in the lens epithelium of cataract patients
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Increased aquaporin 1 and 5 membrane expression in the lens epithelium of cataract patients

机译：白内障患者晶状体上皮中水通道蛋白1和5的膜表达增加

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In this work we have analyzed the expression levels of the main aquaporins (AQPs) expressed in human lens epithelial cells (HLECs) using 112 samples from patients treated with cataract surgery and 36 samples from individuals treated with refractive surgery, with transparent lenses as controls. Aquaporin-1 (AQP1) is the main AQP, representing 64.1% of total AQPs in HLECs, with aquaporin-5 (AQP5) representing 35.9% in controls. A similar proportion of each AQP in cataract was found. Although no differences were found at the mRNA level compared to controls, a significant 1.65-fold increase (p = 0.001) in AQP1 protein expression was observed in HLECs from cataract patients, with the highest differences being found for nuclear cataracts (2.1-fold increase; p < 0.001). A similar trend was found for AQP5 (1.47-fold increase), although the difference was not significant (p = 0.161). Moreover we have shown increased membrane AQP5 protein expression in HLECs of patients with cataracts. No association of AQP1 or AQP5 expression levels with age or sex was observed in either group. Our results suggest regulation of AQP1 and AQP5 at the post-translational level and support previous observations on the implication of AQPI and 5 in maintenance of lens transparency in animal models. Our results likely reflect a compensatory response of the crystalline lens to delay cataract formation by increasing the water removal rate. (C) 2016 Elsevier B.V. All rights reserved.
机译：在这项工作中，我们分析了人晶状体上皮细胞（HLEC）中表达的主要水通道蛋白（AQPs）的表达水平，使用了白内障手术患者的112个样本和屈光手术患者的36个样本，并以透明晶状体为对照。 Aquaporin-1（AQP1）是主要的AQP，占HLEC中总AQP的64.1％，而Aquaporin-5（AQP5）在对照中占35.9％。发现白内障中每种AQP的比例相似。尽管与对照组相比在mRNA水平上没有发现差异，但是在白内障患者的HLEC中观察到AQP1蛋白表达显着增加1.65倍（p = 0.001），其中核白内障的差异最大（增加2.1倍） ; p <0.001）。对于AQP5也发现了类似的趋势（增加了1.47倍），尽管差异并不显着（p = 0.161）。此外，我们显示白内障患者的HLEC中膜AQP5蛋白表达增加。两组均未观察到AQP1或AQP5表达水平与年龄或性别相关。我们的研究结果提示在翻译后水平调控AQP1和AQP5，并支持先前关于AQPI和5对维持动物模型晶状体透明性的影响的观察。我们的结果可能反映了晶状体通过增加脱水率来延迟白内障形成的代偿反应。 （C）2016 Elsevier B.V.保留所有权利。

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