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首页> 外文期刊>Journal of health science. >Decreased Gene Expression of Testicular Cell-Specific Proteins in Cadmium-Induced Acute Testicular Toxicity
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Decreased Gene Expression of Testicular Cell-Specific Proteins in Cadmium-Induced Acute Testicular Toxicity

机译：镉诱导的急性睾丸毒性反应中睾丸细胞特异性蛋白基因表达的降低

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摘要

Cadmium salts induce severe acute testicular necrosis in rodents. We assessed the expression levels of the genes encoding the follicle-stimulating hormone receptor, luteinizing hormone receptor, testis-specific histone 2B, and transition proteins 1 and 2, which are preferentially expressed in Sertoli cells, Leydig cells, spermatocytes, and spermatids, respectively, by reverse transcription (RT)-PCR using total RNA prepared from the whole testes of cadmium chloride (Cd)-administered rats. Spraque Dawley rats at 3, 7, and 12 weeks of age were singly and subcutaneously injected with Cd at doses of 1, 2.5, 5, 10, 15 or 20 mumol/kg. The expression levels of all genes tested were significantly decreased at doses of over 15 umol/kg (3-week-old rats) or over 10 mumol/kg (7- and 12-week-old rats) 96 hr after injection. Histopathological study showed that these dosages of Cd resulted in extensive disruption of the seminiferous tubules and necrosis of testicular cells, while administration of Cd at a dose of 5 mumol/kg in 7-and 12-week-old rats resulted in only partial degeneration of seminiferous tubules and testicular cells. Therefore, their reduced gene expression is likely to serve as an indicator of Cd-induced testicular necrosis accompanied by cell death. In addition, the susceptibility to Cd-induced testicular damage and decreased gene expression was higher in mature (7- and 12-week-old) rats than in immature (3-week-old) rats.
机译：镉盐可导致啮齿动物严重急性睾丸坏死。我们评估了编码卵泡刺激激素受体，促黄体生成激素受体，睾丸特异性组蛋白2B以及过渡蛋白1和2的基因的表达水平，它们分别在Sertoli细胞，Leydig细胞，精母细胞和精子细胞中优先表达。通过使用从氯化镉（Cd）给药大鼠的整个睾丸制备的总RNA进行逆转录（RT）-PCR。在3、7和12周龄的Spraque Dawley大鼠中，分别以1、2.5、5、10、15或20 mumol / kg的剂量和皮下注射Cd。注射后96小时，在超过15 umol / kg（3周龄的大鼠）或超过10μmol/ kg（7周和12周龄的大鼠）的剂量下，所有测试的基因的表达水平均显着降低。组织病理学研究表明，这些剂量的Cd会导致曲细精管的广泛破坏和睾丸细胞坏死，而在7周和12周龄的大鼠中以5μmol/ kg的剂量施用Cd只会导致Cd的部分变性。曲细精管和睾丸细胞。因此，它们降低的基因表达很可能充当Cd诱导的睾丸坏死伴有细胞死亡的指标。此外，成熟（7周和12周龄）大鼠对Cd引起的睾丸损伤和基因表达降低的敏感性高于未成熟（3周龄）大鼠。

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