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首页> 外文期刊>Journal of health science. >Therapeutic Effects of Gyokuheifusan on NC/Nga Mouse Model of Allergic Dermatitis
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Therapeutic Effects of Gyokuheifusan on NC/Nga Mouse Model of Allergic Dermatitis

机译：绞股蓝散对变应性皮炎NC / Nga小鼠模型的治疗作用

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Gyokuheifusan (GHS) is a traditional Chinese medicine (TCM) formula used to treat various allergic diseases; however, there is little information about its clinical efficacy or mechanism of action. We previously showed that GHS down-regulates over-production of IgE and interleukin (IL)-4 in a mouse model of ovalbumin (OVA)-induced asthma, a major Th2-dominant disease. Here, we investigated the effect of GHS in an NC/Nga mouse model of atopic dermatitis (AD) induced by mite antigen. NC/Nga mice were immunized with an intradermal injection of mite antigen extract twice a week from 6 to 12 weeks of age. GHS (3.0g/kg) was orally administered daily to the GHS-treated group for 6 weeks, and dermatitis score and total serum IgE level were measured sequentially. Spleen was harvested at 13 weeks of age, and the levels of cytokines released from splenocytes (interferon (IFN)-gamma, IL-4 and IL-5) and IgGl/IgG2a levels in sera were measured by ELISA. Mice treated with GHS showed milder dermatitis than the disease-control group and the increase of total serum IgE level tended to be suppressed by GHS. In addition, GHS treatment suppressed the production of IL-4 and IgGl, though it did not affect the production of IFN-gamma and IgG2a; i.e., GHS normalized the Thl/Th2 balance. These results suggest that GHS inhibits the development and reduces the severity of AD, at least in this model.
机译：Gyokuheifusan（GHS）是用于治疗各种过敏性疾病的传统中药（TCM）配方;但是，有关其临床疗效或作用机理的信息很少。我们先前显示，GHS在卵清蛋白（OVA）诱导的哮喘（一种主要的Th2占主导地位的疾病）的小鼠模型中下调IgE和白介素（IL）-4的过量生产。在这里，我们调查了GHS在螨虫抗原诱发的特应性皮炎（AD）的NC / Nga小鼠模型中的作用。从6至12周龄每周两次皮内注射螨抗原提取物对NC / Nga小鼠进行免疫。每天口服GHS（3.0g / kg）至GHS治疗组，持续6周，并随后测量皮炎评分和总血清IgE水平。在13周龄时收获脾脏，并通过ELISA测量从脾细胞释放的细胞因子水平（干扰素（IFN）-γ，IL-4和IL-5）和血清中的IgG1 / IgG2a水平。与疾病对照组相比，接受GHS治疗的小鼠表现出较轻的皮炎，并且GHS倾向于抑制总血清IgE水平的升高。另外，GHS处理抑制了IL-4和IgG1的产生，尽管它不影响IFN-γ和IgG2a的产生。即，GHS将Th1 / Th2平衡归一化。这些结果表明，GHS至少在该模型中抑制了AD的发展并降低了AD的严重性。

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