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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Human neutrophils synthesize IL-8 in an IgE-mediated activation.
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Human neutrophils synthesize IL-8 in an IgE-mediated activation.

机译：人类嗜中性粒细胞在IgE介导的激活中合成IL-8。

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摘要

It has been demonstrated that neutrophils are responsible for the release of large amounts of the inflammatory chemokine interleukin-8 (IL-8), associated with inflammation. To further define the mechanisms implicated, we have analyzed the response of human neutrophils from allergic patients to specific antigens or challenge with anti-immunoglobulin (Ig)E antibodies. Neutrophils showed a dose- and time-dependent production of IL-8. The release of the cytokine was parallel to expression of IL-8 mRNA analyzed by the polymerase chain reaction. This expression was transient-it occurred after 3 h of anti-IgE treatment and was maintained for 18 h. Trifluoperazine, EGTA, reduced nicotinamide adenine dinucleotide phosphate-oxidase inhibitors, and reactive oxygen species (ROS) scavengers inhibited IL-8 production, indicating a critical dependence of calcium and oxidative stress. Moreover, an inhibitory effect of cyclosporin A, an immunosuppressor that inhibits calcineurin activity, on IL-8 release and IL-8 mRNA expression was observed. This is the first evidence of the involvement of ROS and calcium/calcineurin in IgE-dependent IL-8 production. These findings open new perspectives into the functional role of neutrophils in IgE-associated diseases.
机译：已经证明中性粒细胞负责释放与炎症相关的大量炎性趋化因子白细胞介素8（IL-8）。为了进一步确定所涉及的机制，我们已经分析了过敏患者的人类嗜中性粒细胞对特定抗原的反应或使用抗免疫球蛋白（Ig）E抗体的攻击。中性粒细胞显示IL-8的剂量和时间依赖性产生。细胞因子的释放与通过聚合酶链反应分析的IL-8 mRNA的表达平行。该表达是瞬时的，它在抗IgE治疗3小时后发生，并保持18小时。 Trifluoperazine，EGTA，减少的烟酰胺腺嘌呤二核苷酸磷酸氧化酶抑制剂和活性氧（ROS）清除剂抑制IL-8产生，表明钙和氧化应激的关键依赖性。此外，观察到环孢菌素A（一种抑制钙调神经磷酸酶活性的免疫抑制剂）对IL-8释放和IL-8 mRNA表达的抑制作用。这是ROS和钙/钙调神经磷酸酶参与IgE依赖性IL-8产生的第一个证据。这些发现为中性粒细胞在IgE相关疾病中的功能作用开辟了新的视角。

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