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首页> 外文期刊>Journal of mass spectrometry: JMS >A high-throughput multicomponent screening method for diuretics, masking agents, central nervous system (CNS) stimulants and opiates in human urine by UPLC-MS/MS
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A high-throughput multicomponent screening method for diuretics, masking agents, central nervous system (CNS) stimulants and opiates in human urine by UPLC-MS/MS

机译：通过UPLC-MS / MS对尿液中的利尿剂，掩蔽剂，中枢神经系统（CNS）兴奋剂和鸦片制剂进行高通量多组分筛选

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摘要

A simple and rapid multicomponent screening method of 130 substances for direct injections of urine samples has been developed. The fully automated method based on ultra-performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS) is used for three different classes of doping agents: diuretics, central nervous system stimulants (CNS stimulants) and opiates. The samples are diluted with buffer containing internal standards (IS) by a pipetting robot system into 96-well plates. Samples are injected on a reversed phase sub 2-mu m particle column connected to a fast polarity switching and rapid scanning tandem mass spectrometer with an electrospray interface. The software used to evaluate the results produced reports containing a small-sized window for each component and a data table list with flags to indicate any adverse analytical findings in the sample. The report can also be processed automatically using an application software, which interpret the data and indicate if there is a suspicious sample. One 96-well plate can be analyzed within 16 h.
机译：已经开发了一种简单快速的用于直接注射尿液样品的130种物质的多组分筛选方法。基于超高效液相色谱（UPLC）和串联质谱（MS / MS）的全自动方法用于三种不同类型的掺杂剂：利尿剂，中枢神经系统兴奋剂（CNS兴奋剂）和鸦片剂。通过移液机器人系统用含有内标（IS）的缓冲液将样品稀释到96孔板中。将样品注入连接到具有电喷雾接口的快速极性切换和快速扫描串联质谱仪的亚2微米以下反相颗粒色谱柱上。用于评估结果的软件生成的报告包含每个组件的小窗口和带有标志的数据表列表，这些标志指示样本中任何不利的分析发现。还可以使用应用程序软件自动处理该报告，该应用程序解释数据并指出是否有可疑样品。可以在16小时内分析一个96孔板。

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