Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification

Drabik A.; Bodzon-Kulakowska A.; Suder P.

首页> 外文期刊>Journal of mass spectrometry: JMS >Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification

【24h】

Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification

机译：ETD / PTR反应在自上而下的蛋白质组学中的应用，是自下而上的nanoLC-MS / MS蛋白质鉴定的更快替代方法

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Our goal was to compare two popular analytical techniques used nowadays in proteomic investigations for proteins/peptides sequencing and identification, a widely used nanoLC-MS/MS approach applied in the bottom-up proteomics and electron transfer dissociation/proton transfer reaction fragmentation preferably used when top-down strategy is applied. Comparison was carried out with the aid of the ESI-quadrupole ion-trap instrument using the following criteria: total time of analysis including sample preparation, sequence coverage, Mascot scoring, capability to detect modifications, quality of the results as a function of protein molecular weight and sample consumption.

机译：我们的目标是比较当今蛋白质组学研究中用于蛋白质/肽测序和鉴定的两种流行分析技术，自下而上蛋白质组学中广泛使用的nanoLC-MS / MS方法和电子转移解离/质子转移反应片段采用自上而下的策略。借助于ESI-四极杆离子阱仪器，使用以下标准进行比较：分析的总时间，包括样品制备，序列覆盖，吉祥物评分，检测修饰的能力，结果质量与蛋白质分子的关系重量和样品消耗。

著录项

来源
《Journal of mass spectrometry: JMS》 |2012年第10期|共6页
作者
Drabik A.; Bodzon-Kulakowska A.; Suder P.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词
electron transfer dissociation; nanoLC-MS/MS; protein identification; proteomics; proton transfer reaction;

机译：电子转移解离;nanoLC-MS / MS;蛋白质鉴定;蛋白质组学;质子转移反应;

相似文献

外文文献
中文文献
专利

1. Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification [J] . Drabik A., Bodzon-Kulakowska A., Suder P. Journal of mass spectrometry: JMS . 2012,第10期

机译：ETD / PTR反应在自上而下的蛋白质组学中的应用，是自下而上的nanoLC-MS / MS蛋白质鉴定的更快替代方法
2. On-Chip Sample Preparation Using a ChipFilter Coupled to NanoLC-MS/MS for Bottom-Up Proteomics [J] . Ndiaye Massamba M., Ha Phuong Ta, Chiappetta Giovanni, Journal of proteome research . 2020,第7期

机译：使用ChopFilter连接到Nanolc-MS / MS的片上样品制备，用于自下而上蛋白质组学
3. Software for top-down proteomic identification of a plasmid-borne factor (and other proteins) from genomically sequenced pathogenic bacteria using MALDI-TOF-TOF-MS/MS and post-source decay [J] . Fagerquist Clifton K., Lee Bertram G., Zaragoza William J., International journal of mass spectrometry . 2019,第期

机译：使用MALDI-TOF-TOF-MS / MS和源极衰减的基因组排序的致病细菌的质粒传播系数（和其他蛋白质）的自上蛋白质组鉴定的软件
4. Top-Down Analysis by LC-MS/MS (ETD) of Spatially-Resolved Tissue Section Extracts for Protein Identification in MALDI Imaging Experiments [C] . Kristie M. Rose, David M. Anderson, Kevin L. Schey American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：通过LC-MS / MS（ETD）的自上而下分析，在空间分离的组织截面提取物中用于MALDI成像实验中的蛋白质鉴定
5. Multidimensional separation of intact proteins for differential proteomics employing top-down and bottom-up proteomic strategies [D] . Richardson, Brenna McJury 2010

机译：使用自上而下和自下而上的蛋白质组学策略对差异蛋白质组学进行完整蛋白质的多维分离
6. Complete Protein Characterization using ETD and PTR with the Combination of Top-Down and Bottom-Up Strategies in an Ion Trap [O] . A. Ingendoh, R. Hartmer, A. Brekenfeld, 2010

机译：使用ETD和PTR结合离子阱中的自上而下和自下而上的策略进行完整的蛋白质表征
7. Ion/ion reactions for top-down proteomics Topology of inner membrane proteins Proteomes of Plasmodium gametocytes Changes in saliva biomarkers over time On-chip protein treatment for SELDI MS Protein preconcentrator Quantitative proteomics with CDITs Glycoprotein analysis SNPs, alternative splicing, and PTMs The missing selenoproteome [O] . 2005

机译：自上而下蛋白质组学的离子/离子反应内膜蛋白的拓扑疟原虫蛋白质蛋白质蛋白质随着时间的推移唾液生物标志物的变化 Seldi MS的片上蛋白质处理蛋白质预融合器定量蛋白质组学与CDITS 糖蛋白分析 SNP，替代拼接和PTMS 失踪的selenoproteome.

Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification

摘要

著录项

相似文献

相关主题

期刊订阅