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Modified MALDI MS fatty acid profiling for bacterial identification

机译:改良的MALDI MS脂肪酸谱用于细菌鉴定

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摘要

Bacterial fatty acid profiling is a well-established technique for bacterial identification. Ten bacteria were analyzed using both positive- and negative-ion modes with a modified matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) approach using CaO as a matrix replacement (metal oxide laser ionization MS (MOLI MS)). The results show that reproducible lipid cleavage similar to thermal in situ tetramethyl ammonium hydroxide saponification/derivatization had occurred. Principal component analysis showed that replicates from each organism grouped in a unique space. Cross validation (CV) of spectra from both ionization modes resulted in greater than 94% validation of the data. When CV results were compared for the two ionization modes, negative-ion data produced a superior outcome. MOLI MS provides clinicians a rapid, reproducible and cost-effective bacterial diagnostic tool.
机译:细菌脂肪酸谱分析是一种用于细菌鉴定的成熟技术。使用正离子和负离子两种模式,使用改良的基质辅助激光解吸电离质谱(MALDI MS)方法(使用CaO作为基质替代物)(金属氧化物激光电离MS(MOLI MS))分析了10种细菌。结果显示发生了类似于热原位氢氧化四甲基铵皂化/衍生化的可再现的脂质裂解。主成分分析表明,来自每个生物的复制品在一个独特的空间中分组。来自两种电离模式的光谱的交叉验证(CV)导致数据验证超过94%。当比较两种电离模式的CV结果时,负离子数据产生了更好的结果。 MOLI MS为临床医生提供了一种快速,可重复且经济高效的细菌诊断工具。

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