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首页> 外文期刊>Journal of mass spectrometry: JMS >Analytical characterization of microfabricated SU-8 emitters for electrospray ionization mass spectrometry
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Analytical characterization of microfabricated SU-8 emitters for electrospray ionization mass spectrometry

机译：用于电喷雾电离质谱分析的SU-8微型发射器的分析表征

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We present a detailed optimization and characterization of the analytical performance of SU-8-based emitters for electrospray ionization mass spectrometry (ESI/MS). The improved SU-8 fabrication process presented here enhances patterning accuracy and reduces the time and cost of fabrication. All emitters are freestanding and enable sample delivery by both pressure-driven and spontaneous flows. The optimized emitter design incorporates a sharp, double-cantilevered tip implemented to the outlet of an SU-8 microchannel and provides highly sensitive ESI/MS detection. Moreover, the optimized design allows the use of relatively large microchannel dimensions (up to 200 x 50 mu m(2), W x h) without sacrificing the detection sensitivity. This is advantageous with a view of preventing emitter clogging and enabling reproducible analysis. The measured limits of detection for the optimized emitter design were 1 nm for verapamil and 4 nm for Glu-fibrinopeptide B with good quantitative linearities between 1 nm and 10 mu m (R-2 = 0.9998) for verapamil and between 4 nm and 3 mu m (R-2 = 0.9992) for Glu-fibrinopeptide B. The measured tip-to-tip repeatability for signal intensity was 14% relative standard deviation (RSD) (n = 3; 5 mu m verapamil) and run-to-run repeatability 4-11% RSD (n = 4; 5 gm verapamil) for all individual emitters tested. In addition, long-term stability of < 2% RSD was maintained for timescales of 30 min even under free flow conditions. SU-8 polymer was also shown to be chemically stable against most of the tested electrospray solvents.
机译：我们介绍了基于SU-8的发射器的电喷雾电离质谱（ESI / MS）的分析性能的详细优化和特性。本文介绍的改进的SU-8制造工艺可提高图案形成的准确性，并减少制造时间和成本。所有的发射器都是独立式的，可以通过压力驱动和自发流进行样品输送。优化的发射器设计在SU-8微通道的出口处采用了尖锐的双悬臂式尖端，并提供了高度灵敏的ESI / MS检测。而且，优化的设计允许使用相对较大的微通道尺寸（最大200 x 50μm（2），W x h），而不会牺牲检测灵敏度。考虑到防止发射极堵塞并且能够进行可重复的分析，这是有利的。优化的发射体设计的检测限为维拉帕米1 nm和Glu-纤维蛋白肽B 4 nm，维拉帕米1 nm至10μm（R-2 = 0.9998）和4 nm至3 mu之间具有良好的定量线性对于Glu-纤维蛋白肽B，m（R-2 = 0.9992）。测得的信号强度的点对点重复性为14％相对标准偏差（RSD）（n = 3; 5μm维拉帕米），并且每次运行对于所有测试的发射器，重复性为4-11％RSD（n = 4; 5 gm维拉帕米）。此外，即使在自由流动的条件下，在30分钟的时间范围内也保持了<2％RSD的长期稳定性。还显示出SU-8聚合物对大多数测试的电喷雾溶剂具有化学稳定性。

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