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首页> 外文期刊>Journal of mass spectrometry: JMS >Identification of compounds from high-fat and extra virgin olive oil-supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry
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Identification of compounds from high-fat and extra virgin olive oil-supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry

机译：使用质谱法鉴定全脂肝和分离的线粒体中高脂和初榨橄榄油补充饮食中的化合物

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摘要

Nonalcoholic steatohepatitis (NASH) is a fatty liver disorder that could be improved with extra virgin olive oil (EVOO) supplementation in diet. We propose the monitoring, in whole mouse liver extracts and in isolated mitochondria, of the absorption of compounds from three different diets: standard (CT), high-fat (HFD) and high-fat supplemented with EVOO (HFSO). Male mice were submitted to one of the following three diets: CT or HFD for 16 weeks or HFD for 8 weeks followed by additional 8 weeks with HFSO. Following this period, liver was extracted for histological evaluation, mitochondria isolation and mass spectrometry analyses. Diets, liver extracts and Percoll-purified mitochondria were analyzed using ESI-MS and the lipidomics approach. Morphological, histological and spectrometric results indicated a decrease in NASH severity with EVOO supplementation in comparison with animals maintained with HFD. Spectrometric data also demonstrated that some compounds presented on the diets are absorbed by the mitochondria. EVOO was shown to be a potential therapeutic alternative in food for NASH. Our results are in accordance with the proposition that the major factor that influences different responses to diets is their composition - and not only calories - especially when it comes to studies on obesity. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：非酒精性脂肪性肝炎（NASH）是一种脂肪肝疾病，可以通过在饮食中添加特级初榨橄榄油（EVOO）来改善。我们建议在整个小鼠肝脏提取物中和分离的线粒体中监测来自三种不同饮食的化合物的吸收：标准饮食（CT），高脂饮食（HFD）和补充EVOO（HFSO）的高脂肪饮食。雄性小鼠接受以下三种饮食之一：CT或HFD进行16周或HFD进行8周，然后再用HFSO进行8周。在此期间之后，提取肝脏进行组织学评估，线粒体分离和质谱分析。使用ESI-MS和脂质组学方法分析饮食，肝脏提取物和Percoll纯化的线粒体。形态学，组织学和光谱学结果表明，与维持HFD的动物相比，补充EVOO可降低NASH严重程度。光谱数据还表明，饮食中存在的某些化合物被线粒体吸收。 EVOO被证明是NASH食品中潜在的治疗替代品。我们的研究结果与以下命题一致，即影响饮食不同反应的主要因素是其成分-不仅是卡路里-特别是在肥胖研究方面。版权所有（C）2015 John Wiley＆Sons，Ltd.

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