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首页> 外文期刊>Journal of mass spectrometry: JMS >Changes in lipid distribution in E. coli strains in response to norfloxacin
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Changes in lipid distribution in E. coli strains in response to norfloxacin

机译：响应诺氟沙星的大肠杆菌菌株中脂质分布的变化

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Bacterial resistance to antibiotics has become an increasing threat, requiring not only the development of new targets in drug discovery, but more importantly, a better understanding of cellular response. In the current study, three closely related Escherichia coli strains, a wild type (MG1655) and an isogenic pair derived from the wild type (DPB635 and DPB636) are studied following exposure to sub lethal concentrations of antibiotic (norfloxacin) over time. In particular, genotype similarities between the three strains were assessed based on the lipid regulation response (e.g. presence/absence and up/down regulation). Lipid identification was performed using direct surface probe analysis (matrix-assisted laser desorption/ionization, MALDI), coupled to high-resolution mass spectrometry (Fourier transform ion cyclotron resonance mass spectrometry, FT-ICR MS) followed by statistical analysis of variability and reproducibility across batches using internal standards. Inspection of the lipid profile showed that for the MG1655, DPB635 and DPB636 E. coli strains, a similar distribution of the altered lipids was observed after exposure to norfloxacin antibiotic (e.g. fatty acids and glycerol phospholipids are up and down regulated, respectively). Additionally, variations in the lipid distribution resemble the extent to which each strain can combat the antibiotic exposure. That is, the topA66 topoisomerase I mutation of DPB636 translates into diminished response related to antibiotic sensitivity when compared to MG1655 and the DPB635 strains. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：细菌对抗生素的耐药性已成为日益严重的威胁，不仅需要开发新的药物靶标，而且更重要的是，需要更好地了解细胞反应。在当前的研究中，随着时间的推移，暴露于亚致死浓度的抗生素（诺氟沙星）后，研究了三种密切相关的大肠杆菌菌株，即野生型（MG1655）和源自野生型的同基因对（DPB635和DPB636）。特别地，基于脂质调节反应（例如，存在/不存在和上/下调节）来评估三个菌株之间的基因型相似性。使用直接表面探针分析（基质辅助激光解吸/电离，MALDI），高分辨率质谱（Fourier变换离子回旋共振质谱，FT-ICR MS）和随后的变异性和重现性统计分析，进行脂质鉴定使用内部标准跨批次进行。脂质分布图的检查表明，对于MG1655，DPB635和DPB636大肠杆菌菌株，在暴露于诺氟沙星抗生素后（例如分别上调和下调了脂肪酸和甘油磷脂），观察到了类似的脂质变化分布。另外，脂质分布的变化类似于每种菌株可以抵抗抗生素暴露的程度。也就是说，与MG1655和DPB635菌株相比，DPB636的topA66拓扑异构酶I突变转化为与抗生素敏感性相关的应答减弱。版权所有（c）2015 John Wiley＆Sons，Ltd.

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