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首页> 外文期刊>Journal of mass spectrometry: JMS >Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A
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Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A

机译：表面活化化学电离和阳离子交换色谱法分析肠毒素A

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摘要

Surface-activated chemical ionization (SACI) has been widely used in recent years for the analysis of different compounds (e.g. peptides, street drugs, amino acids). The main benefits of this technology are its high sensitivity and its effectiveness under different chromatographic conditions [i.e. ion exchange chromatography and reversed-phase (RP) chromatography]. Here we used SACI in conjunction with quadrupole time-of-flight mass spectrometry to analyze enterotoxin A, which is produced by Staphylococcus aureus, in milk matrix using both RP and ion exchange chromatographies. SACI had increased sensitivity as compared with electrospray ionization. Moreover, the higher quantitation efficiency of this technique, mainly in terms of limit of detection (0.01 ng/ml), limit of quantitation (0.05 ng/ml), linearity range (0.05-50 ng/ml), matrix effect, accuracy (intraday and interday accuracy errors were 9.2% and 10.3%, respectively) and precision (intraday and interday precision errors were 5.3% and 12.8%, respectively), is shown and discussed.
机译：近年来，表面活化化学电离（SACI）已被广泛用于分析不同的化合物（例如肽，街头毒品，氨基酸）。该技术的主要优点是它的高灵敏度和在不同色谱条件下的有效性。离子交换色谱和反相（RP）色谱]。在这里，我们将SACI与四极杆飞行时间质谱结合使用，通过RP和离子交换色谱法分析了牛奶基质中金黄色葡萄球菌产生的肠毒素A。与电喷雾电离相比，SASI具有更高的灵敏度。此外，该技术的定量效率更高，主要体现在检测限（0.01 ng / ml），定量限（0.05 ng / ml），线性范围（0.05-50 ng / ml），基质效应，准确性（显示并讨论了日内和日间精度误差分别为9.2％和10.3％）和精度（日内和日间精度误差分别为5.3％和12.8％）。

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