...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >TLR9 and TLR7/8 activation induces formation of keratic precipitates and giant macrophages in the mouse cornea
【24h】

TLR9 and TLR7/8 activation induces formation of keratic precipitates and giant macrophages in the mouse cornea

机译：TLR9和TLR7 / 8激活诱导在小鼠角膜中形成角质沉淀物和巨噬细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Macrophage adherence to the inner corneal surface and formation of MGCs in the stroma are common signs of chronic inflammation following corneal infection. To determine whether macrophage adherence (known clinically as KPs) and giant cell formation were specific to innate immune activation via particular TLR ligands, macrophage activation was examined in a murine model of TLR-mediated corneal inflammation. The corneal epithelium was debrided and highly purified TLR ligands were topically applied once to the cornea of TLR72/2, TLR9(-/-), Cx(3)cr1(gfp/+), CD11c(eYFP), and IL-4(-/-) mice. At 1 week post-treatment macrophage activation and phenotype was evaluated in the cornea. Treatment with TLR2, TLR3, TLR4, and TLR5 ligands caused an increase in the number of activated stromal macrophages in the central cornea at 1 week post-treatment. However, treatment with TLR9 ligand CpG-ODN and the TLR7/8 ligand R848/Resiquimod led to an accumulation of macrophages on the corneal endothelium and formation of multinucleated giant macrophages in the corneal stroma. We suggest that giant cell formation, which is a characteristic feature of granuloma formation in many tissues, may be a unique feature of TLR9-and TLR7/8-mediated macrophage activation.
机译：巨噬细胞粘附于角膜内表面并在基质中形成MGC是角膜感染后慢性炎症的常见征兆。为了确定巨噬细胞的粘附（临床上称为KP）和巨细胞形成是否特定于通过特定TLR配体的先天免疫激活，在TLR介导的角膜炎症的鼠模型中检查了巨噬细胞的激活。清创角膜上皮，将高纯度的TLR配体局部施用于TLR72 / 2，TLR9（-/-），Cx（3）cr1（gfp / +），CD11c（eYFP）和IL-4（ -/-） 老鼠。治疗后1周，评估角膜中巨噬细胞的活化和表型。在治疗后1周，用TLR2，TLR3，TLR4和TLR5配体进行治疗可导致角膜中央活化基质巨噬细胞数量增加。然而，用TLR9配体CpG-ODN和TLR7 / 8配体R848 /瑞西莫特治疗导致巨噬细胞在角膜内皮上积累并在角膜基质中形成多核巨噬细胞。我们建议巨细胞形成，这是肉芽肿在许多组织中形成的一个特征，可能是TLR9和TLR7 / 8介导的巨噬细胞激活的独特特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号