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首页> 外文期刊>Journal of Medical Entomology >Discrimination of Culicoides Midge Larvae Using Multiplex Polymerase Chain Reaction Assays Based on DNA Sequence Variation at the Mitochondrial Cytochrome C Oxidase I Gene
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Discrimination of Culicoides Midge Larvae Using Multiplex Polymerase Chain Reaction Assays Based on DNA Sequence Variation at the Mitochondrial Cytochrome C Oxidase I Gene

机译：基于线粒体细胞色素C氧化酶I基因DNA序列变异的多重聚合酶链反应分析法区分Culicoides ge幼虫

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摘要

The recent spread of Bluetongue disease in northwestern Europe has indicated the ability of Palaearctic Culicoides species to vector the disease. Because the different midge species vary in their ability to harbor and transmit the Bluetongue virus, quick and reliable identification is necessary to resolve the species composition of midge communities, both adult and larval, at any place at any given time point. Given that morphological identification of Culicoides species is problematic, we developed three, multiplex polymerase chain reaction (PCR) assays that facilitate high-throughput analysis of midge specimens. One assay distinguishes between species of the so-called Culicoides obsoletus s.l. complex (including C. dewulfi), whereas two assays facilitate differentiation of species W of the Culicoides pulicaris s.l. complex, These assays yield two PCR products: one species-specific and one generic band. We show the application of the assays in the analysis of Culicoides larvae from three different farms in northeast Scotland.
机译：蓝舌病在欧洲西北部的最近传播表明古库蚊物种具有传播这种疾病的能力。由于不同的蚊种具有藏匿和传播蓝舌病毒的能力，因此，有必要进行快速而可靠的鉴定，以解决在任何给定时间点在任何地方的成年和幼体蚊虫群落的物种组成。鉴于Culicoides物种的形态鉴定存在问题，我们开发了三种多重聚合酶链反应（PCR）分析方法，可帮助对中标本进行高通量分析。一种测定法区分所谓的Culicoides obsoletus s.l.的种。复杂（包括C. dewulfi），而两种测定法有助于区分Culicoides pulicaris s.l.的W种。这些测定法产生两种PCR产物：一种物种特异性和一种通用谱带。我们展示了该分析方法在分析苏格兰东北部三个不同农场的Culicoides幼虫中的应用。

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