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首页> 外文期刊>Journal of Microbiological Methods >Design and validation of a novel quantitative method for rapid bacterial enumeration using programmed stage movement scanning electron microscopy
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Design and validation of a novel quantitative method for rapid bacterial enumeration using programmed stage movement scanning electron microscopy

机译：设计和验证一种新颖的定量细菌快速定量方法的程序化的台架移动扫描电子显微镜

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摘要

The adhesion of bacteria to surgical implants is the first stage of implant infection. The method for detecting bound bacteria is an important consideration in the study of bacterial adherence and colonisation. Enumeration of bacteria by direct visualisation techniques is labour intensive and time consuming. We have developed and validated a method for enumerating bacteria on porous material surfaces using programmed stage movement scanning electron microscopy and compared cumulative counts after 1-10 stage movements with absolute bacterial counts. We describe this method with three commercially sourced meshes used for abdominal wall hernia repair and with three different inoculums of Staphylococcus epidermidis. The results demonstrate significant correlation to the absolute count after five cumulative counts for all meshes analysed. The mean time saved by the cumulative counting method was 1 h and 9 min per mesh. We conclude that advances in scanning electron microscopy and the advent of precise automated stage control have facilitated rapid data acquisition for bacterial counting purposes and that five cumulative counts at 1000 x or 2500 x magnification are a valid quantitative method for enumerating S. epidermidis bacteria on porous surfaces (with a pore size of up to 13 mm)
机译：细菌对外科植入物的粘附是植入物感染的第一步。检测结合细菌的方法是研究细菌粘附和定殖的重要考虑因素。通过直接可视化技术计数细菌是费力且费时的。我们已经开发并验证了一种使用程序台移动扫描电子显微镜对多孔材料表面上的细菌进行计数的方法，并将1-10次移动后的累积计数与绝对细菌计数进行了比较。我们用三种用于腹壁疝修补的商业来源的网片和表皮葡萄球菌的三种不同接种物描述了这种方法。结果表明，对所有网格进行五次累积计数后，其与绝对计数之间存在显着相关性。累积计数方法节省的平均时间为每个网格1小时9分钟。我们得出结论，扫描电子显微镜的进步和精确的自动化载物台控制的出现促进了细菌计数目的的快速数据采集，并且在1000 x或2500 x放大倍数下的五个累积计数是枚举多孔上表皮葡萄球菌的有效定量方法。表面（最大孔径为13毫米）

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