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Z-spectroscopy with Alternating-Phase Irradiation

机译:交替照射的Z光谱

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Magnetization transfer (MT) MRI and Z-spectroscopy are tools to study both water-macromolecule interactions and pH-sensitive exchange dynamics between water and the protons of mobile chemical groups within these macromolecules. Both rely on saturation of frequencies offset from water and observation of the on-resonance water signal. In this work, an RF saturation method called Z-spectroscopy with Alternating-Phase Irradiation (ZAPI) is introduced. Based on the T2-selectivity of the irradiation pulse, ZAPI can be used to separate the different contributions to a Z-spectrum, as well as to study the T2 distribution of the macromolecules contributing to the MT signal. ZAPI can be run at resonance for water and with low power, thus minimizing problems with specific absorption rate (SAR) limits in clinical applications. In this paper, physical and practical aspects of ZAPI are discussed and the sequence is applied in vitro to sample systems and in vivo to rat head to demonstrate the method.
机译:磁化传递(MT)MRI和Z光谱学是研究水与大分子相互作用以及水与这些大分子内可移动化学基团的质子之间的pH敏感交换动力学的工具。两者都依赖于偏离水的频率的饱和度以及对共振水信号的观察。在这项工作中,介绍了一种称为Z光谱和交替相辐射(ZAPI)的RF饱和方法。基于辐照脉冲的T2选择性,ZAPI可用于分离对Z光谱的不同贡献,以及研究对MT信号有贡献的大分子的T2分布。 ZAPI可以与水共振并以低功率运行,从而最大程度地减少了临床应用中特定吸收率(SAR)限制的问题。在本文中,讨论了ZAPI的物理和实用方面,并将该序列体外应用于样品系统,体内应用于大鼠头部以证明该方法。

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