...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Virology >Low prevalence of HPV in Brazilian children with retinoblastoma.
【24h】

Low prevalence of HPV in Brazilian children with retinoblastoma.

机译：巴西儿童视网膜母细胞瘤HPV患病率低。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Retinoblastoma is the most frequent intra-ocular malignant tumor of the childhood, occurring in 1 of 18,000-30,000 live births. Little is known about the causes of sporadic retinoblastoma and only a few authors have investigated the etiologic role of human papillomavirus (HPV), with controversial results. Formalin-fixed, paraffin-embedded tissue blocks containing retinoblastoma were retrieved from the archives of the Department of Pathology at Hospital A C Camargo, Sao Paulo, Brazil. All patients were treated with enucleation (21 children had both eyes enucleated). Retinoblastoma and, when possible, normal retina of each specimen, were micro-dissected under direct light microscopic visualization by using a PixCell II Laser Capture Micro-dissection System. The DNA quality was evaluated by polymerase chain reaction (PCR) amplification of 110 base pairs fragment of the human beta-globin gene using primers PCO3+/PCO4+. All globin positive specimens were analyzed by PCR for the presence of HPV DNA using consensus primers GP5+/GP6+. A total of 154 specimens were evaluated. Forty-four patients also had normal retinal specimens available for analysis of DNA HPV. The DNA HPV prevalence among all tumor specimens was 4.6% (95% CI 2.0; 8.8) (7 positive specimens/153 adequate specimens). Among normal retinal specimens, the DNA HPV prevalence was 9.1% (95% CI 2.9; 20.5) (4 positive specimens/44 specimens). There was no statistically significant difference between these rates (P = 0.318). Excluding any experimental failure, our results indicate a low prevalence of HPV DNA in retinoblastomas. We were therefore unable to conclude about the association between these oncogenic viruses and this rare pediatric neoplasm.
机译：视网膜母细胞瘤是儿童期最常见的眼内恶性肿瘤，在18,000-30,000例活产中有1例发生。关于散发性视网膜母细胞瘤的病因知之甚少，只有少数作者调查了人乳头瘤病毒（HPV）的病因学作用，并引起了争议。从巴西圣保罗A C Camargo医院病理科的档案中检索到福尔马林固定，石蜡包埋的组织，其中含有视网膜母细胞瘤。所有患者均接受摘除术（21名儿童的双眼摘除）。通过使用PixCell II Laser Capture Micro-dissection System在直接光学显微镜下对视网膜母细胞瘤和每个标本的正常视网膜进行显微解剖。使用引物PCO3 + / PCO4 +，通过聚合酶链反应（PCR）扩增人β-珠蛋白基因的110个碱基对片段来评估DNA质量。使用共有引物GP5 + / GP6 +，通过PCR分析所有球蛋白阳性标本中是否存在HPV DNA。总共评估了154个样本。四十四名患者也有可用于分析DNA HPV的正常视网膜标本。所有肿瘤样本中的DNA HPV患病率为4.6％（95％CI 2.0; 8.8）（7阳性样本/ 153适当样本）。在正常的视网膜样本中，DNA HPV患病率为9.1％（95％CI 2.9; 20.5）（4阳性样本/ 44样本）。这些比率之间没有统计学上的显着差异（P = 0.318）。排除任何实验失败，我们的结果表明在视网膜母细胞瘤中HPV DNA的患病率较低。因此，我们无法就这些致癌病毒与这种罕见的儿科肿瘤之间的联系得出结论。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号