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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Vibrational circular dichroism spectra of protein films: thermal denaturation of bovine serum albumin
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Vibrational circular dichroism spectra of protein films: thermal denaturation of bovine serum albumin

机译：蛋白质膜的振动圆二色性光谱：牛血清白蛋白的热变性

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摘要

Vibrational circular dichroism (VCD) spectroscopy has been used for the first time to investigate the thermal denaturation of proteins in H2O solutions. Films prepared from heated aqueous solutions were used for these investigations. A well-known alpha-helical protein, bovine serum albumin (BSA), is used for this first study. Both VCD and infrared absorption results obtained for BSA films indicate that the heat treatment of BSA induces significant amounts of beta-sheet structure and that the denaturation process is irreversible. To verify the irreversible nature of thermal denaturation, optical rotation was also measured as a function of temperature in both heating and cooling cycles. These results also indicate that thermal denaturation of BSA in solution is irreversible. This study establishes the usefulness of films for VCD investigations and offers new avenues for VCD studies on biologically important systems. (C) 2004 Elsevier B.V. All rights reserved.
机译：振动圆二色性（VCD）光谱已首次用于研究H2O溶液中蛋白质的热变性。由加热的水溶液制备的膜用于这些研究。这项研究使用了众所周知的α螺旋蛋白牛血清白蛋白（BSA）。 BSA膜获得的VCD和红外吸收结果均表明，BSA的热处理会诱导大量的β-折叠结构，并且变性过程是不可逆的。为了验证热变性的不可逆性，还测量了加热和冷却循环中随温度变化的旋光度。这些结果还表明溶液中BSA的热变性是不可逆的。这项研究建立了电影对VCD研究的有用性，并为重要生物学系统的VCD研究提供了新途径。 （C）2004 Elsevier B.V.保留所有权利。

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