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首页> 外文期刊>Clinical oral implants research >Bio-degradation of a resorbable collagen membrane (Bio-Gide) applied in a double-layer technique in rats.
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Bio-degradation of a resorbable collagen membrane (Bio-Gide) applied in a double-layer technique in rats.

机译：在大鼠中应用双层技术对可吸收胶原膜（Bio-Gide）进行生物降解。

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OBJECTIVE: The aim of this study was to evaluate histologically the bio-degradation of two layers of Bio-Gide((R)) (BG) membrane, as compared with that of a single layer. MATERIAL AND METHODS: Two circular calvarial bony defects, 5 mm in diameter, were made in 24 Wistar rats. BG membrane, labeled with biotin, was cut into 5-mm-diameter disks, and placed in defects either as a mono-layer membrane (MLM) or as a double-layer membrane (DLM). Rats were sacrificed after 4 or 9 weeks and histology was performed. Membranes were stained with horseradish peroxidase-conjugated streptavidin and aminoethyl carbazole as a substrate for detection of biotinylated collagen. The area of collagen and thickness of the residual membranes were measured by image analysis software. Statistical analysis was performed using the non-parametric Wilcoxon's signed-ranks test. RESULTS: At 4-week collagen area per measurement window within the DLM sites (0.09+/-0.05 mm(2)) was significantly greater (P<0.01) than that in the MLM sites (0.047+/-0.034 mm(2)). At 9 weeks, the collagen area was also greater in the DLM sites (0.037+/-0.026 mm(2)) compared with that of the MLM sites (0.025+/-0.016 mm(2)); however, this difference did not reach statistical significance. The rate of membrane degradation, calculated as percent membrane lost compared with baseline, was similar for the DLM and MLM at both time points ( approximately 60% at 4 weeks and approximately 80% at 9 weeks). In addition, the residual DLM thickness at 4 weeks (475.5+/-73.77 mum) was significantly (P<0.01) greater than that of MLM (262.38+/-48.01 mum). At 9 weeks, membrane thickness was also greater in the DLM sites (318.22+/-70.45 mum) compared with that of the MLM sites (183.32+/-26.72 mum); however, this difference did not reach statistical significance. The reduction in thickness between 4 and 9 weeks was 30% for MLM and 33% for DLM. DISCUSSION: The use of a double layer of BG membrane results in a barrier of increased collagen area and thickness, compared with application of a single layer.
机译：目的：本研究的目的是从组织学上评估两层Bio-Gide（B）膜与单层膜相比的生物降解。材料与方法：在24只Wistar大鼠中制作了两个直径5 mm的圆形颅骨骨缺损。将标记有生物素的BG膜切成5毫米直径的圆盘，并以单层膜（MLM）或双层膜（DLM）的形式放置在缺陷中。 4或9周后处死大鼠，并进行组织学检查。用辣根过氧化物酶结合的链霉亲和素和氨乙基咔唑作为底物对膜进行染色，以检测生物素化的胶原蛋白。通过图像分析软件测量胶原蛋白的面积和残留膜的厚度。使用非参数Wilcoxon的秩和检验进行统计分析。结果：在DLM部位内每个测量窗口的4周胶原面积（0.09 +/- 0.05 mm（2））显着大于PLM（0.01），在MLM部位（0.047 +/- 0.034 mm（2）） ）。在9周时，与MLM部位（0.025 +/- 0.016 mm（2））相比，DLM部位（0.037 +/- 0.026 mm（2））的胶原蛋白面积也更大;但是，这种差异没有达到统计学意义。在两个时间点，DLM和MLM的膜降解率（以膜相对于基线的损失百分比计算）相似（4周时约为60％，9周时约为80％）。另外，在第4周的残余DLM厚度（475.5 +/-73.77μm）显着（P <0.01）大于MLM（262.38 +/-48.01μm）。在9周时，与MLM部位（183.32 +/- 26.72微米）相比，DLM部位（318.22 +/- 70.45微米）的膜厚度也更大;但是，这种差异没有达到统计学意义。对于MLM，在4至9周之间厚度减少了30％，对于DLM，减少了33％。讨论：与单层施用相比，双层BG膜的使用导致增加胶原蛋白面积和厚度的障碍。

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