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首页> 外文期刊>Journal of Molecular Biology >Promoter-specific involvement of the FixJ receiver domain in transcriptional activation
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Promoter-specific involvement of the FixJ receiver domain in transcriptional activation

机译：FixJ受体域在转录激活中的启动子特异性参与

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摘要

The "two-component" FixLJ system activates nitrogen fixation genes via nifA and fixK in Sinorhizobium meliloti. Like other response regulators, the FixJ protein can be decomposed into an N-terminal phosphorylatable "receiver" domain FixJN and a C-terminal transcriptional activator domain FixJC. The FixJN receiver domain was known to regulate activity of FixJC negatively at the nifA promoter. Here we show a different situation at the fixK promoter where FixJN also contributes positively to transcriptional activation. This promoter-specific effect was mapped by alanine-scanning mutagenesis to the beta2 strand of the receiver domain. This interaction with FixJN is required for the recruitment of RNA polymerase at the fixK promoter by phosphorylated FixJ. Altogether the FixJ receiver domain appears to carry at least four functions, some of which can be separated by mutation: (1) autophosphorylation; (2) inhibition of FixJC; (3) dimerization; (4) transcriptional activation at pfixK. This example illustrates the formidable functional plasticity of receiver domains.
机译：“两组分” FixLJ系统通过nifA和fixK激活苜蓿中华根瘤菌中的固氮基因。像其他响应调节器一样，FixJ蛋白可以分解为N端可磷酸化的“受体”结构域FixJN和C端转录激活结构域FixJC。已知FixJN受体域在nifA启动子上负调控FixJC的活性。在这里，我们在fixK启动子处显示了不同的情况，其中FixJN也对转录激活起积极作用。通过丙氨酸扫描诱变将该启动子特异性作用定位到受体结构域的β2链。与FixJN的这种相互作用是通过磷酸化FixJ在fixK启动子处募集RNA聚合酶所必需的。 FixJ接收器域似乎总共具有至少四个功能，其中一些功能可以通过突变分开：（1）自磷酸化; （2）抑制FixJC; （3）二聚化; （4）pfixK的转录激活。此示例说明了接收域的强大功能可塑性。

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